Rob G. J. M. van der Lans

TL;DR: In this article, the authors used the Compartment Model Approach (CMA) to develop a flow model based on the general knowledge of the hydrodynamics of both unaerated and aerated stirred vessels.

TL;DR: The simulations show that at present the decisive factor for a successful integration of fluid dynamics and microbial kinetics is the kinetics, and that the biomass prediction overestimated the biomass concentration.

TL;DR: It was suggested that additional interactions, such as steric/brush effects, play an important role in the aggregation process and as a result selective flotation of PHA IBs was observed near this pH.

TL;DR: The enzymatic disruption process gives a higher product release than homogenization with or without ammonia pretreatment at lower operating costs, but it also yields a much smaller cell debris size than the other disruption process, which is unfavorable for centrifugal inclusion body purification.

TL;DR: Almost foamless Escherichia coli fermentations were realised using the design of a 30,000 l scale bioreactor using an up-pumping hydrofoil impeller and a mechanistic model for SAFD, which proved to be scalable.