Journal ArticleDOI
Mixing in large-scale vessels stirred with multiple radial or radial and axial up-pumping impellers: modelling and measurements
TLDR
In this article, the authors used the Compartment Model Approach (CMA) to develop a flow model based on the general knowledge of the hydrodynamics of both unaerated and aerated stirred vessels.About:
This article is published in Chemical Engineering Science.The article was published on 2000-12-01. It has received 198 citations till now. The article focuses on the topics: Slip factor & Impeller.read more
Citations
More filters
Journal ArticleDOI
Living with heterogeneities in bioreactors: understanding the effects of environmental gradients on cells.
TL;DR: In this review, the theoretical analyses that predict the existence of environmental gradients in bioreactors and their experimental confirmation are reviewed and studies based on the scale-down methodology, a convenient tool for assessing the effect of environmental heterogene ities, are surveyed.
Journal ArticleDOI
The translation of cell-based therapies: clinical landscape and manufacturing challenges.
Thomas R.J. Heathman,Alvin W. Nienow,Alvin W. Nienow,Mark J.S. McCall,Karen Coopman,Bo Kara,Christopher J. Hewitt,Christopher J. Hewitt +7 more
TL;DR: The aim of this study is to inform industry and academia of current cell-based therapy clinical development and to identify gaps in their manufacturing requirements.
Journal ArticleDOI
Studies related to the scale-up of high-cell-density E. coli fed-batch fermentations using multiparameter flow cytometry: effect of a changing microenvironment with respect to glucose and dissolved oxygen concentration.
Christopher J. Hewitt,Gerhard Nebe-Von Caron,Britta Axelsson,Caroline M. McFarlane,Alvin W. Nienow +4 more
TL;DR: Flow cytometric analysis of cells in broth samples has revealed, for the first time, that a progressive change in cell physiological state generally occurs throughout the course of fed-batch bacterial fermentations, and has demonstrated that a changing microenvironment with respect to substrate concentration has a profound effect on cell physiology and hence on viable biomass yield.
Book ChapterDOI
The Scale‐Up of Microbial Batch and Fed‐Batch Fermentation Processes
TL;DR: The ability to obtain data on how a recombinant laboratory process may perform at the large scale, dependent on feeding regime employed or controlling action taken is invaluable for any detailed and informed development program.
Journal ArticleDOI
Transcriptional and metabolic response of recombinant Escherichia coli to spatial dissolved oxygen tension gradients simulated in a scale-down system.
Alvaro R. Lara,Lidia Leal,Noemí Flores,Guillermo Gosset,Francisco Bolívar,Octavio T. Ramírez +5 more
TL;DR: Differences in transcription levels between aerobic and anaerobic compartments were observed, indicating that E. coli can respond very fast to intermittent DOT conditions and are useful for establishing rational scale-up criteria and strain design strategies for improved culture performance at large scales.
References
More filters
Book
Basic Bioreactor Design
TL;DR: Based on a graduate course in biochemical engineering, provides the basic knowledge needed for the efficient design of bioreactors and the relevant principles and data for practical process engineering with an emphasis on enzyme reactors and aerated reactors for microorganisms.
Book
Fluid mixing and gas dispersion in agitated tanks
TL;DR: In this paper, the essentials of fluid mechanics and how to apply fundamental principles to achieve optimum time and capacity efficiency with a minimum of waste are discussed, and guidelines for performing design calculations, determining design limits and validating design methodology are provided.
Journal ArticleDOI
On impeller circulation and mixing effectiveness in the turbulent flow regime
TL;DR: In this article, an equation for mixing time recently published by BHR Group can be related to a basic turbulence model and it is also shown that this equation is superior to those based on a theory linking mixing time to the flow generated by the impeller.
Journal ArticleDOI
Substrate gradient formation in the large-scale bioreactor lowers cell yield and increases by-product formation
TL;DR: IPTG induction of a recombinant protein was shown to influence important cell parameters and considerably increased the yield of carbon dioxide per glucose added, indicating an increased maintenance in the large-scale bioreactor.
Related Papers (5)
Physiological responses to mixing in large scale bioreactors
Sven-Olof Enfors,Mehmedalija Jahic,A. Rozkov,Bo Xu,Michael Hecker,Britta Jürgen,Elke Krüger,Thomas Schweder,G. Hamer,D. O'Beirne,Naruemol Noisommit-Rizzi,Matthias Reuss,L. Boone,Christopher J. Hewitt,Caroline M. McFarlane,Alvin W. Nienow,Tamas Kovacs,Christian Trägårdh,Laszlo Fuchs,Johan Revstedt,P. C. Friberg,Bjørn H. Hjertager,G. Blomsten,Harald Skogman,S. Hjort,Frans W. J. M. M. Hoeks,H. Y. Lin,Peter Neubauer,R. G. J. M. van der Lans,Karel Ch. A. M. Luyben,Peter Vrábel,A. Manelius +31 more