Robert P.C. Shiu

TL;DR: Chick embryo fibroblasts transformed by Rous sarcoma virus have an increased content of two membrane proteins of molecular weights 78,000 and 95,000 which may have an important role in regulating the utilization of glucose in cultured cells.

TL;DR: The induction of these two proteins by glucose starvation suggests that they have a role in glucose utilization, and two glucose derivatives, glucosamine and 2-deoxyglucose, are known to interfere with the glycosylation process.

TL;DR: Pulsechase experiments indicate that this 56,000 dalton molecular weight protein is extremely unstable, with a half-life of about 20 min, and does not serve as a precursor to any of the detectable virion polypeptides.

TL;DR: Immunofluorescence studies using affinity purified antibodies to GRP-78 revealed that this protein was not exposed on the cell surface but was localized in a granular vesicular network inside the cell that resembles the distribution of endoplasmic reticulum.

TL;DR: The addition of epidermal growth factor and insulin to cultures of normal chicken heart mesenchymal cells causes these cells to proliferate at a rate comparable to that of their RSV-infected counterparts.