Robyn Joyce Russell

TL;DR: In vitro expression of two natural and two synthetic chimeric alleles shows that the Asp137 substitution alone is responsible for both the loss of E3's carboxylesterase activity and the acquisition of a novel OP hydrolase activity.

TL;DR: The structure of OPDA is reported and regions of the protein that are likely to give it a preference for substrates that have shorter alkyl substituents are identified.

TL;DR: The early outbreak of resistance in this species can be explained by the preexistence of mutant alleles encoding malathion resistance, and the pinned specimen analysis shows much higher genetic diversity at the locus before organophosphate use, suggesting that the subsequent sweep of diazinon resistance in Australasia has compromised the scope for the loci to respond further to the ongoing challenge of the insecticides.

TL;DR: The Est-6 gene of Drosophila melanogaster was cloned by screening libraries with synthetic oligonucleotides corresponding to tryptic peptides from purified esterase-6 (Est-6) protein to reveal a protein of 548 residues with 19% sequence similarity to acetylcholinesterase from the Torpedo ray.

TL;DR: In this paper, a comparison of the genomic and cDNA sequences revealed that the Est-6 gene comprises two exons, one of 1,387 bp and one of 248 bp, separated by a short intron of 51 bp.