S. Xu

TL;DR: The first high-viscosity injector-based SMX experiments carried out at a US synchrotron source, the Advanced Photon Source (APS), are reported.

TL;DR: In a systematic and direct experimental demonstration of reduced radiation damage in protein crystals with small beams, damage was measured as a function of micron-sized X-ray beams of decreasing dimensions and is less anisotropic than photoelectrons emission probability, consistent with photoelectron trajectory simulations.

TL;DR: A unique triple-collimator apparatus, which has been in routine use on both undulator beamlines since February 2008, allows users to rapidly interchange the focused beam and conditioned mini-beams of two sizes with a single mouse click, has greatly facilitated sample screening and resulted in several structures that could not have been obtained with the larger focused beam.

TL;DR: An X-ray mini-beam of 8 × 6 µm cross-section was used to collect diffraction data from protein microcrystals with volumes as small as 150–300‵m3 to investigate the benefits of this technique for experiments with small crystals and with large inhomogeneous crystals.

TL;DR: The protocol covers preparation of in situ plates and setup of crystallization trials; 3D printing and assembly of holders; opening of plates, isolation of film patches containing crystals, and loading them onto holders; basic screening and data-collection guidelines; and unloading of holders, as well as reuse and recycling of them.