Chelsie E. Conrad

TL;DR: The crystal structure of a constitutively active form of human rhodopsin bound to a pre-activated form of the mouse visual arrestin is determined by serial femtosecond X-ray laser crystallography and provides a basis for understanding GPCR-mediated arrestin-biased signalling.

TL;DR: Time resolved experiments on PSII nano/microcrystals from Thermosynechococcus elongatus performed with the recently developed technique of serial femtosecond crystallography provide evidence that PSII undergoes significant conformational changes at the electron acceptor side and at the Mn4CaO5 core of the OEC.

TL;DR: This work used microcrystals of photoactive yellow protein as a model system and obtained high-resolution, time-resolved difference electron density maps of excellent quality with strong features, which open the way to the study of reversible and nonreversible biological reactions on time scales as short as femtoseconds under conditions that maximize the extent of reaction initiation throughout the crystal.

TL;DR: Structural changes associated with the earliest steps in the trans-to-cis isomerization of the chromophore in photoactive yellow protein are identified.

TL;DR: The recently developed method of serial femtosecond crystallography at an X-ray free-electron laser determined the room-temperature crystal structure of the human AT(1)R in complex with its selective antagonist ZD7155 at 2.9-Å resolution, and revealed key structural features of AT( 1)R and critical interactions for ZD 7155 binding.