Sabine Kupzig

TL;DR: It is shown that the final stage of reticulocyte maturation occurs by a previously undescribed mechanism in which large glycophorin A-containing vesicles forming at the cytosolic face of the plasma membrane are internalized and fuse with autophagosomes before expulsion of theAutophagosomal contents by exocytosis.

TL;DR: It is demonstrated a feasible approach to the manufacture of red cells for clinical use from in vitro culture, immortalizing early adult erythroblasts generating a stable line, which provides a continuous supply ofred cells.

TL;DR: Using an optimized ex vivo differentiation system for human erythropoiesis, it is observed that maturing reticulocytes are characterized by the presence of one or few large vacuolar compartments, and it is speculated that autophagy is needed to concentrate the final remnants of the Reticulocyte endomembrane system in autophagosome/endosome hybrid compartments that are primed to undergo exocytosis.

TL;DR: Protein sorting during human erythroblast enucleation using fluorescence activated cell sorting (FACS) was studied to obtain pure populations of reticulocytes and nuclei produced by in vitro culture to propose that the membrane proteins lost with the nucleus represent an excess mobile population of either individual proteins or protein complexes.

TL;DR: Knockdown of RhAG during early erythropoiesis was accompanied by a concomitant drop in membrane expression of Rh polypeptides, consistent with assembly of major components of the band 3 macrocomplex at an early stage during erythrocyte membrane assembly.