Sage McKeand

TL;DR: A peptide (P2C) is identified that mediates transduction of Cas9 RNP from the female hemolymph to the developing mosquito oocytes, resulting in heritable gene editing of the offspring with efficiency as high as 0.3 mutants per injected mosquito.

TL;DR: The ReMOT Control technique is adapted to deliver Cas9 ribonucleoprotein complex to adult mosquito ovaries, generating targeted and heritable mutations in the malaria vector Anopheles stephensi without injecting embryos, opening the power of CRISPR/Cas9 methods to malaria laboratories that lack the equipment or expertise.

TL;DR: The adaptation of this technology to Anopheles mosquitoes opens up the power of reverse genetics to malaria vector labs that do not have the equipment or technical expertise to perform embryo injections and establishes the flexibility of ReMOT Control for gene-editing in non-Aedes species.

TL;DR: In this article, the authors presented novel genome assemblies for the North American species An. crucians, An. freeborni, An albimanus, and An. quadrimaculatus, and examined the evolutionary relationship between these species.

TL;DR: In this article , the authors presented genome assemblies for Anopheles crucians, freeborni, albimanus, and quadrimaculatus and examined the evolutionary relationship between these species.