Sam Swift

TL;DR: A reliable affinity purification strategy to identify specific interactors that combines quantitative SILAC-based mass spectrometry with characterization of common contaminants binding to affinity matrices (bead proteomes) is presented.

TL;DR: A quantitative Förster resonance energy transfer-based methodology using multiphoton fluorescence lifetime imaging microscopy was developed and found that under homeostatic conditions, the interaction between Keap1 and Nrf2 follows a cycle in which the complex sequentially adopts two distinct conformations.

TL;DR: FRET analysis of cell lines expressing fluorescently tagged histones on separate nucleosomes demonstrates that variations in chromosome compaction occur during mitosis.

TL;DR: Sds22 defines protein phosphatase 1 location and function at kinetochores and subsequent activity of aurora B in mitosis.

TL;DR: The discovery of the isothiocyanate sulforaphane as a potent inducer which reacts with cysteine sensors of Keap1, leading to activation of Nrf2, and the experimental evidence for the “cyclic sequential attachment and regeneration” or “conformation cycling” model of KeAP1-mediated NRF2 degradation is presented.