S
Samuel T. Hess
Researcher at University of Maine
Publications - 75
Citations - 11142
Samuel T. Hess is an academic researcher from University of Maine. The author has contributed to research in topics: Microscopy & Photoactivated localization microscopy. The author has an hindex of 28, co-authored 68 publications receiving 10278 citations. Previous affiliations of Samuel T. Hess include Yale University & National Institutes of Health.
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Journal ArticleDOI
Ultra-High Resolution Imaging by Fluorescence Photoactivation Localization Microscopy
TL;DR: A new method for fluorescence imaging has been developed that can obtain spatial distributions of large numbers of fluorescent molecules on length scales shorter than the classical diffraction limit, and suggests a means to address a significant number of biological questions that had previously been limited by microscope resolution.
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Imaging coexisting fluid domains in biomembrane models coupling curvature and line tension
TL;DR: It is shown that high-resolution fluorescence imaging using two dyes preferentially labelling different fluid phases directly provides a correlation between domain composition and local membrane curvature, which is able to provide experimental estimates of boundary tension between fluid bilayer domains.
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Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples.
Manuel F. Juette,Travis J. Gould,Mark D. Lessard,Michael J. Mlodzianoski,Bhupendra S. Nagpure,Brian Bennett,Samuel T. Hess,Jörg Bewersdorf +7 more
TL;DR: A light microscope that generates images with translationally invariant 30 × 30 × 75nm resolution over a depth of several micrometers enabling 3D sub-diffraction resolution without compromising speed or sensitivity is reported.
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Large-scale fluid/fluid phase separation of proteins and lipids in giant plasma membrane vesicles
Tobias Baumgart,Adam T. Hammond,Prabuddha Sengupta,Samuel T. Hess,David Holowka,Barbara Baird,Watt W. Webb +6 more
TL;DR: It is demonstrated that giant plasma membrane vesicles (GPMVs) or blebs formed from the plasma membranes of cultured mammalian cells can also segregate into micrometer-scale fluid phase domains, and GPMVs now provide an effective approach to characterize biological membrane heterogeneities.
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Biological and Chemical Applications of Fluorescence Correlation Spectroscopy: A Review†
TL;DR: Technological advances in detectors, autocorrelation electronics, and confocal microscopy were incorporated into FCS, and the ability of FCS to resolve multiple species with equivalent diffusion properties was extended by probability analysis of fluorescence intensity distributions.