M
Manuel F. Juette
Researcher at Cornell University
Publications - 20
Citations - 2328
Manuel F. Juette is an academic researcher from Cornell University. The author has contributed to research in topics: Ribosome & Translation (biology). The author has an hindex of 15, co-authored 18 publications receiving 2006 citations. Previous affiliations of Manuel F. Juette include Heidelberg University & Yale University.
Papers
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Journal ArticleDOI
Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples.
Manuel F. Juette,Travis J. Gould,Mark D. Lessard,Michael J. Mlodzianoski,Bhupendra S. Nagpure,Brian Bennett,Samuel T. Hess,Jörg Bewersdorf +7 more
TL;DR: A light microscope that generates images with translationally invariant 30 × 30 × 75nm resolution over a depth of several micrometers enabling 3D sub-diffraction resolution without compromising speed or sensitivity is reported.
Journal ArticleDOI
Ultra-stable organic fluorophores for single-molecule research
Qinsi Zheng,Manuel F. Juette,Steffen Jockusch,Michael R. Wasserman,Zhou Zhou,Roger B. Altman,Scott C. Blanchard +6 more
TL;DR: Self-healing organic fluorophores, wherein the triplet state is intramolecularly quenched by a covalently attached protective agent, exhibit markedly improved photostabilities and are likely to impact the future of single-molecule research.
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Single-molecule analysis of ligand efficacy in β2AR–G-protein activation
G. Glenn Gregorio,Matthieu Masureel,Daniel Hilger,Daniel S. Terry,Manuel F. Juette,Hong Zhao,Zhou Zhou,Jose Manuel Perez-Aguilar,Maria Hauge,Signe Mathiasen,Jonathan A. Javitch,Harel Weinstein,Brian K. Kobilka,Scott C. Blanchard +13 more
TL;DR: It is shown that partial and full agonists differentially affect TM6 motions to regulate the rate at which GDP-bound β2AR–Gs complexes are formed and the efficiency of nucleotide exchange leading to Gs activation, which sheds new light on the G-protein activation mechanism.
Journal ArticleDOI
Single-molecule imaging of non-equilibrium molecular ensembles on the millisecond timescale
Manuel F. Juette,Daniel S. Terry,Michael R. Wasserman,Roger B. Altman,Zhou Zhou,Hong Zhao,Scott C. Blanchard +6 more
TL;DR: A single-molecule imaging and analysis platform using scientific complementary metal-oxide semiconductor (sCMOS) detectors that enables imaging of 15,000 individual molecules simultaneously at millisecond rates enabled the detection of previously obscured processes relevant to the fidelity mechanism in protein synthesis.
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Experimental characterization of 3D localization techniques for particle-tracking and super-resolution microscopy.
TL;DR: A localization algorithm that is independent of theoretical models and therefore generally applicable to a large number of experimental realizations is introduced and the performance of the two major 3D techniques based on astigmatic distortions and on multiplane detection is compared.