Journal ArticleDOI
Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples.
Manuel F. Juette,Travis J. Gould,Mark D. Lessard,Michael J. Mlodzianoski,Bhupendra S. Nagpure,Brian Bennett,Samuel T. Hess,Jörg Bewersdorf +7 more
TLDR
A light microscope that generates images with translationally invariant 30 × 30 × 75nm resolution over a depth of several micrometers enabling 3D sub-diffraction resolution without compromising speed or sensitivity is reported.Abstract:
Imaging volumes as thick as whole cells at three-dimensional (3D) super-resolution is required to reveal unknown features of cellular organization. We report a light microscope that generates images with translationally invariant 30 x 30 x 75 nm resolution over a depth of several micrometers. This method, named biplane (BP) FPALM, combines a double-plane detection scheme with fluorescence photoactivation localization microscopy (FPALM) enabling 3D sub-diffraction resolution without compromising speed or sensitivity.read more
Citations
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Journal ArticleDOI
Super-Resolution Fluorescence Microscopy
TL;DR: It is anticipated that super-resolution fluorescence microscopy will become a widely used tool for cell and tissue imaging to provide previously unobserved details of biological structures and processes.
Journal ArticleDOI
Fluorescent probes for super-resolution imaging in living cells
TL;DR: The contributions of fluorescent probes to far-field super-resolution imaging, focusing on fluorescent proteins and organic small-molecule fluorophores are described, to reach the goal of video-rate imaging of live cells with molecular resolution.
Journal ArticleDOI
A guide to super-resolution fluorescence microscopy
TL;DR: These new super-resolution technologies are either based on tailored illumination, nonlinear fluorophore responses, or the precise localization of single molecules and have created unprecedented new possibilities to investigate the structure and function of cells.
Journal ArticleDOI
Nanomedicine--challenge and perspectives.
Kristina Riehemann,Stefan W. Schneider,Thomas A. Luger,Biana Godin,Mauro Ferrari,Harald Fuchs +5 more
TL;DR: This Review gives an overview of selected recent developments and applications of nanomedicine.
Journal ArticleDOI
Breaking the Diffraction Barrier: Super-Resolution Imaging of Cells
TL;DR: This Primer explains the principles of various super-resolution approaches, such as STED, (S)SIM, and STORM/(F)PALM, and demonstrates how these approaches are beginning to provide new insights into cell biology, microbiology, and neurobiology.
References
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Journal ArticleDOI
Numerical Recipes, The Art of Scientific Computing
Journal ArticleDOI
Imaging intracellular fluorescent proteins at nanometer resolution.
Eric Betzig,George H. Patterson,Rachid Sougrat,O. Wolf Lindwasser,Scott G. Olenych,Juan S. Bonifacino,Michael W. Davidson,Jennifer Lippincott-Schwartz,Harald F. Hess +8 more
TL;DR: This work introduced a method for optically imaging intracellular proteins at nanometer spatial resolution and used this method to image specific target proteins in thin sections of lysosomes and mitochondria and in fixed whole cells to image retroviral protein Gag at the plasma membrane.
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Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM).
TL;DR: A high-resolution fluorescence microscopy method based on high-accuracy localization of photoswitchable fluorophores that can, in principle, reach molecular-scale resolution is developed.
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Ultra-High Resolution Imaging by Fluorescence Photoactivation Localization Microscopy
TL;DR: A new method for fluorescence imaging has been developed that can obtain spatial distributions of large numbers of fluorescent molecules on length scales shorter than the classical diffraction limit, and suggests a means to address a significant number of biological questions that had previously been limited by microscope resolution.
Journal ArticleDOI
Far-Field Optical Nanoscopy
TL;DR: Initial applications indicate that emergent far-field optical nanoscopy will have a strong impact in the life sciences and in other areas benefiting from nanoscale visualization.