Sandii N. Brimble

TL;DR: The observation that hESC lines maintained in vitro develop genetic and epigenetic alterations implies that periodic monitoring of these lines will be required before they are used in in vivo applications and that some late-passage hESS lines may be unusable for therapeutic purposes.

TL;DR: These studies identify previously unappreciated RTKs that support hESC proliferation and self-renewal, and provide a rationally designed medium for the growth and maintenance of pluripotent hESCs.

TL;DR: In this paper, the earliest available cryopreserved stocks of the BG01, BG02, and BG03 hESC lines were recovered and expanded, which exhibited multiple definitive characteristics of undifferentiated cells, including long-term self-renewal, expression of markers of pluripotency, maintenance of a normal karyotype, and differentiation to mesoderm, endoderm and ectoderm.

TL;DR: Recovered and expanded the earliest available cryopreserved stocks of the BG01, BG02, and BG03 hESC lines, which exhibited multiple definitive characteristics of undifferentiated cells, including long-term self-renewal, expression of markers of pluripotency, and maintenance of a normal karyotype.

TL;DR: It is demonstrated that the GSLs recognized as SSEA‐3 and ‐4 do not play critical functional roles in maintaining the pluripotency of hESCs, but instead suggested roles for this class of molecules during cellular differentiation.