Sandra N. Pinto

TL;DR: The results suggest that generation of different ceramide species in cell membranes has a distinct biophysical impact with acyl chain saturation dictating membrane lateral organization, and chain asymmetry governing interdigitation and membrane morphology.

TL;DR: The application of an established fluorescence multiprobe approach, together with x-ray diffraction, differential scanning calorimetry, and confocal fluorescence microscopy, allowed the characterization of NCer and the determination of the phase diagram of palmitoyloleoylphosphatidylcholine (POPC)/NCer binary mixtures.

TL;DR: Fluorescence lifetime imaging microscopy shows that the synthesized symmetric cationic molecule with D-π-A(+)-π-D architecture is also able to penetrate within the nucleus.

TL;DR: The results show an increase in the order of cellular membranes in CerS2-transfected cells caused by the enrichment in very long acyl chain SLs, which reinforces the significance of Cer-induced changes on membrane biophysical properties as a likely molecular mechanism by which different acylChain Cers exert their specific biological actions.

TL;DR: The results suggest that in biological membranes, lipid domains such as rafts and ceramide platforms, might display distinctive biophysical properties depending on the local lipid composition at the site of the membrane where they are formed, further highlighting the potential role of membraneBiophysical properties as an underlying mechanism for mediating specific biological processes.