S
Satpal Virdee
Researcher at University of Dundee
Publications - 33
Citations - 1736
Satpal Virdee is an academic researcher from University of Dundee. The author has contributed to research in topics: Ubiquitin & Ubiquitin ligase. The author has an hindex of 13, co-authored 27 publications receiving 1352 citations. Previous affiliations of Satpal Virdee include Laboratory of Molecular Biology & Medical Research Council.
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Journal ArticleDOI
Engineered diubiquitin synthesis reveals Lys29-isopeptide specificity of an OTU deubiquitinase
TL;DR: The crystal structure of Lys6-linked diubiquitin is solved, providing a molecular basis for the different biological functions this linkage may regulate, and it is discovered that TRABID cleaves the Lys29 linkage 40-fold more efficiently than the Lys63 linkage.
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Screening of DUB activity and specificity by MALDI-TOF mass spectrometry
Maria Stella Ritorto,Richard Ewan,Ana B. Perez-Oliva,Axel Knebel,Sara J. Buhrlage,Melanie Wightman,Sharon M. Kelly,Nicola T. Wood,Satpal Virdee,Nathanael S. Gray,Nicholas A. Morrice,Dario R. Alessi,Matthias Trost +12 more
TL;DR: This work has developed a sensitive and fast assay to quantify in vitro DUB enzyme activity using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, and confirms the high specificity of many members of the OTU and JAB/MPN/Mov34 metalloenzyme DUB families.
Journal ArticleDOI
Traceless and site-specific ubiquitination of recombinant proteins.
Satpal Virdee,Prashant Kapadnis,Thomas S. Elliott,Kathrin Lang,Julia Madrzak,Duy P. Nguyen,Lutz Riechmann,Jason W. Chin +7 more
TL;DR: This work combines the genetically directed incorporation of 7 with native chemical ligation and desulfurization to yield an entirely native isopeptide bond between substrate proteins and ubiquitin.
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Activity-based E3 ligase profiling uncovers an E3 ligase with esterification activity
Kuan-Chuan Pao,Nicola T. Wood,Axel Knebel,Karim Rafie,Mathew Stanley,Peter D. Mabbitt,Ramasubramanian Sundaramoorthy,Kay Hofmann,Daan M. F. van Aalten,Satpal Virdee +9 more
TL;DR: Non-lysine ubiquitination activity of the E3 ubiquitin ligase MYCBP2 is identified by activity-based profiling and biochemical and structural analysis of MYC BP2 suggests the basis for its mechanism and specificity, and suggests that E3 enzymes have an unappreciated mechanistic diversity.
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Molecular basis for ubiquitin and ISG15 cross-reactivity in viral ovarian tumor domains.
TL;DR: The viral OTU domain of CCHFV (vOTU) is characterized biochemically and structurally, revealing that it hydrolyzes four out of six tested Ub linkages, but lacks activity against linear and K29-linked Ub chains.