Showing papers by "Seongwon Seo published in 2012"

Cobalt ion-mediated cysteine detection with a hyperbranched conjugated polyelectrolyte as a new sensing platform.

Abstract: A highly efficient colorimetric and fluorescence turn-off probe for the sensitive and selective detection of the biologically important amino acid, cysteine (Cys), is demonstrated using a newly synthesized water-soluble hyperbranched polymer (HP) containing sulfonic acid groups. The detection mechanism involves two steps: (i) the slight quenching of HP in the presence of Co(2+) in advance; and (ii) the gradual quenching of the HP-Co(2+) complex according to the concentration of Cys due to the absorption screening effect of the formation of the Cys-Co(2+) complex, which prevents HP from absorbing excitation energy.

Identification of Candidate Genes Associated with Beef Marbling Using QTL and Pathway Analysis in Hanwoo (Korean Cattle)

Abstract: Marbling from intramuscular fat is an important trait of meat quality and has an economic benefit for the beef industry. Quantitative trait loci (QTL) fine mapping was performed to identify the marbling trait in 266 Hanwoo steers using a 10K single nucleotide polymorphism panel with the combined linkage and linkage disequilibrium method. As a result, we found nine putative QTL regions for marbling: three on BTA6, two on BTA17, two on BTA22, and two on BTA29. We detected candidate genes for marbling within 1 cM of either side of the putative QTL regions. Additionally, to understand the functions of these candidate genes at the molecular level, we conducted a functional categorization using gene ontology and pathway analyses for those genes involved in lipid metabolism or fat deposition. In these putative QTL regions, we found 95 candidate genes for marbling. Using these candidate genes, we found five genes that had a direct interaction with the candidate genes. We also found SCARB1 as a putative candidate gene for marbling that involves fat deposition related to cholesterol transport.

Fluorometric detection of lectin with water-soluble hyperbranched conjugated polymer using mannose mediation.

Abstract: A water-soluble hyperbranched polymer containing boronic acid groups at the ends of the polymer, which are capable of binding to diol-containing mannose, was syntheized. The hyperbranched polymer was prepared by a palladium-catalyzed Suzuki cross-coupling reaction using the tribromo monomer for the hyperbranched type structure. The water-soluble hyperbranched polymer (HP) exhibited enhanced fluorescence intensity upon exposure to lectin in the presence of mannose compared to other proteins, such as lysozyme and cytochrome c, because mannose plays a key role in binding both lectin and HP resulting in selective sensing toward lectin.

Effects of different parities on productive performance of lactating sows

Abstract: Fast recovery of feed intake and milk production are very important in the management of lactating sows because they are directly related to sow and litter performance It is also known that parity is one of factors affecting feed intake and milk yield during lactation Therefore, the objective of this study was to evaluate effects of different parities on productive performance of lactating sows fed diets containing different levels of DDGS A total of 245 sows were divided into 3 parity groups of 1 to 2, 3 to 5, and over 5 parities Within each parity group, sows were allottedto 1 of 4 dietary treatments that were prepared by inclusion of 0, 10, 20, and 30% DDGS in lactation diets Diets were fed to sows during lactationSows with parities 1-2 had lower (p ＜ 005) initial sow body weight, sow feed intake, sow weight change, and sow backfat change during lactation than sows with parities 3-5 as well as parities over 5 However, sows with parities over 5 had lower (p ＜ 005) litter size at weaning, litter birth weight, litter weaning weight, and piglet average daily gain and higher pre-weaning mortality than sows with parities 1-2 as well as parities 3-5 In conclusion, parity influences productive performance of lactating sows

Effects of supplementing distillers dried grains with solublesin the diet of lactating sows onvariation in the body weight of piglets

Abstract: Distillers dried grains with solubles (DDGS) is a by-product from processing corn to produce bio-ethanol, and it contains almost three times higher protein, fat, and fiber than corn. Intake of higher amount of nutrients originated from corn in lactating sows may contribute to changes in milk composition and thus alter the growth of suckling piglets. Therefore, the objective of this study was to evaluate effect of supplementingDDGS in the diet of lactating sows on variation in the body weight of piglets. A total of 50 sows with parities of 3 to 5 were usedand allottedinto one of four treatments which included 0 (CON), 10% (DDGS10), 20% (DDGS20), or 30% (DDGS30) DDGS in the diet. Diets were fed to sows during the whole lactation period.Individual body weights of piglets were measured within 24 h after farrowing and at weaning. No significant differences were observed inthe coefficient of variation (CV) for body weight of piglets within a litter after farrowing and at weaning among the treatments (p>0.05). There was, however, a tendency of decreases in the CV for body weight of piglets within a litter at weaning compared with that after farrowingin the DDGS10 and DDGS20 treatments. At weaning, the percentage of the number of piglets weighing less than 4.5 kg in DDGS20 (4.86%, 7 out of 144; p<0.05) or DDGS30 (5.04%, 6 out of 119; p=0.059) was lower than that in CON (12.41%; 17 out of 137) although this pattern was not observed in DDGS10. In conclusion, addition of DDGS in lactating sow diets improved piglet performance by reducing the number piglets weighing less than 4.5 ㎏.

Problems in methodology for estimating cost of milk production and its improvement

Abstract: Accurate estimation of milk production cost is very important for dairy farmers in establishing strategies for business management (e.g. planning a program for milk production, deciding the size of business and investment, determining the milk price for sale). Since the estimated cost of milk production is used as an important index to determine the basal price of milk in Korea, there has been much interest and debate on the method used to estimate milk production cost among the stakeholder. This study was thus carried out to identify problems in the current methodology for estimating cost of milk production, and to find a better way to improve it. We propose several alternatives and better ways to improve the current method for estimating cost of milk production. Estimation of the income and cost per head should be based on the number of cattle converted to grown cows. Cost estimation per liter of milk should be made for both whole milk and 3.4% milk fat corrected milk. The value of purchased cows and raised replacement heifers should be the same as their market value. The productive life span of cows should be less 4 years, and the terminal or salvage value of cows needs to be 30 to 40% less than her initial value. When calculating depreciation of cows over the productive life span, however, the salvage value should be 0 or 1 Korean won. On calculating labor costs, the farm labor wage corresponding to the average wage of nonfarm industrial workers should be assumed. Beside of these, better estimation procedures for other items are also given. The proposed methods from this study should improve the accuracy of estimation of milk production cost and help to achieve consensus among the stakeholder.