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Sergey Brezgin
Researcher at I.M. Sechenov First Moscow State Medical University
Publications - 28
Citations - 372
Sergey Brezgin is an academic researcher from I.M. Sechenov First Moscow State Medical University. The author has contributed to research in topics: Hepatitis B virus & CRISPR. The author has an hindex of 7, co-authored 19 publications receiving 152 citations.
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Journal ArticleDOI
Dead Cas Systems: Types, Principles, and Applications.
TL;DR: Current CRISPR systems used for developing different dCas-based molecular approaches and their most significant applications are described and comments on the state-of-art in theCRISPR field and future directions are made.
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Orthologous CRISPR/Cas9 systems for specific and efficient degradation of covalently closed circular DNA of hepatitis B virus.
Dmitry Kostyushev,Sergey Brezgin,Anastasiya Kostyusheva,Dmitry Zarifyan,Irina A. Goptar,Vladimir Chulanov +5 more
TL;DR: St CRISPR/Cas9 system represents the safest system with high anti-HBV activity, suggesting its utility in targeting many HBV variants, but two or more mismatches impaired or blocked CRISpr/ Cas9 activity, indicating that host DNA will not likely be targeted.
Journal ArticleDOI
Suppressing the NHEJ pathway by DNA-PKcs inhibitor NU7026 prevents degradation of HBV cccDNA cleaved by CRISPR/Cas9.
Dmitry Kostyushev,Anastasiya Kostyusheva,Sergey Brezgin,Dmitry Zarifyan,Anastasiya Utkina,Irina A. Goptar,Vladimir Chulanov +6 more
TL;DR: It is concluded that CRISPR/Cas9 is a highly effective tool to degrade ccc DNA and first demonstrate that inhibiting NHEJ impairs cccDNA degradation.
Posted ContentDOI
CRISPR-Cas Systems for Diagnosing Infectious Diseases
Anastasiya Kostyusheva,Sergey Brezgin,Yurii Babin,Irina Vasil'eva,Dmitry Kostyushev,Vladimir Chulanov +5 more
TL;DR: This review focuses on CRISPR-based technologies and their perspectives to become platforms for point-of-care nucleic acid detection methods and as deployable diagnostic platforms that could help to identify and curb outbreaks and emerging epidemics.
Journal ArticleDOI
CRISPR-Cas systems for diagnosing infectious diseases.
Anastasiya Kostyusheva,Sergey Brezgin,Yurii Babin,Irina Vasilyeva,Dieter Glebe,Dmitry Kostyushev,Vladimir Chulanov +6 more
TL;DR: A review on CRISPR-based technologies and their perspectives to become platforms for point-of-care nucleic acid detection methods and as deployable diagnostic platforms that could help to identify and curb outbreaks and emerging epidemics is presented in this paper.