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Shinsuke Ohnuki
Researcher at University of Tokyo
Publications - 52
Citations - 1001
Shinsuke Ohnuki is an academic researcher from University of Tokyo. The author has contributed to research in topics: Cell morphology & Saccharomyces cerevisiae. The author has an hindex of 16, co-authored 44 publications receiving 691 citations.
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Journal ArticleDOI
Virtual-freezing fluorescence imaging flow cytometry
Hideharu Mikami,Makoto Kawaguchi,Chun-Jung Huang,Chun-Jung Huang,Hiroki Matsumura,Takeaki Sugimura,Kangrui Huang,Cheng Lei,Shunnosuke Ueno,Taichi Miura,Takuro Ito,Kazumichi Nagasawa,Takanori Maeno,Hiroshi Watarai,Hiroshi Watarai,Mai Yamagishi,Sotaro Uemura,Shinsuke Ohnuki,Yoshikazu Ohya,Yoshikazu Ohya,Hiromi Kurokawa,Satoshi Matsusaka,Satoshi Matsusaka,Chia-Wei Sun,Yasuyuki Ozeki,Keisuke Goda +25 more
TL;DR: An optomechanical imaging method is presented that overcomes the trade-off by virtually freezing the motion of flowing cells on the image sensor to effectively achieve 1000 times longer exposure time for microscopy-grade fluorescence image acquisition.
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Intelligent image-activated cell sorting 2.0
Akihiro Isozaki,Hideharu Mikami,Hiroshi Tezuka,Hiroki Matsumura,Kangrui Huang,Marino Akamine,Kotaro Hiramatsu,Takanori Iino,Takuro Ito,Hiroshi Karakawa,Yusuke Kasai,Yan Li,Yuta Nakagawa,Shinsuke Ohnuki,Tadataka Ota,Yong Qian,Shinya Sakuma,Takeichiro Sekiya,Yoshitaka Shirasaki,Nobutake Suzuki,Ehsen Tayyabi,Tsubasa Wakamiya,Muzhen Xu,Mai Yamagishi,Haochen Yan,Qiang Yu,Sheng Yan,Dan Yuan,Wei Zhang,Yaqi Zhao,Fumihito Arai,Robert E. Campbell,Robert E. Campbell,Christophe Danelon,Dino Di Carlo,Dino Di Carlo,Kei Hiraki,Yu Hoshino,Yoichiroh Hosokawa,Mary Inaba,Atsuhiro Nakagawa,Yoshikazu Ohya,Yoshikazu Ohya,Minoru Oikawa,Sotaro Uemura,Yasuyuki Ozeki,Takeaki Sugimura,Nao Nitta,Keisuke Goda +48 more
TL;DR: Equipped with the improved capabilities, this new generation of the iIACS technology holds promise for diverse applications in immunology, microbiology, stem cell biology, cancer biology, pathology, and synthetic biology.
Journal ArticleDOI
Single-cell phenomics reveals intra-species variation of phenotypic noise in yeast
Gaël Yvert,Shinsuke Ohnuki,Satoru Nogami,Yasutaka Imanaga,Steffen Fehrmann,Joseph Schacherer,Yoshikazu Ohya +6 more
TL;DR: It is demonstrated that phenotypic noise does differ quantitatively between natural populations, which supports the possibility that, if noise is adaptive, microevolution may tune it in the wild.
Journal ArticleDOI
Vanillin Inhibits Translation and Induces Messenger Ribonucleoprotein (mRNP) Granule Formation in Saccharomyces cerevisiae: Application and Validation of High-Content, Image-Based Profiling
TL;DR: It is suggested that vanillin represses translation in yeast cells by inhibiting ribosomal function in non-essential yeast deletion mutants.
Journal ArticleDOI
Multiple Functional Domains of the Yeast l,3-β-Glucan Synthase Subunit Fks1p Revealed by Quantitative Phenotypic Analysis of Temperature-Sensitive Mutants
Hiroki Okada,Mitsuhiro Abe,Masayo Asakawa-Minemura,Aiko Hirata,Hiroshi Qadota,Kana Morishita,Shinsuke Ohnuki,Satoru Nogami,Yoshikazu Ohya +8 more
TL;DR: Analysis of quantitative cell morphology and biochemical properties of 10 different temperature-sensitive mutants of FKS1, a putative catalytic subunit of GS, suggest that Fks1p consists of multiple domains that are required for cell wall construction and cellular morphogenesis.