S
Shinya Saijo
Researcher at KEK
Publications - 26
Citations - 533
Shinya Saijo is an academic researcher from KEK. The author has contributed to research in topics: Protein subunit & ATPase. The author has an hindex of 12, co-authored 26 publications receiving 433 citations. Previous affiliations of Shinya Saijo include Tokyo University of Science.
Papers
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Journal ArticleDOI
Rotation mechanism of Enterococcus hirae V1-ATPase based on asymmetric crystal structures
Satoshi Arai,Shinya Saijo,Kano Suzuki,Kenji Mizutani,Kenji Mizutani,Kenji Mizutani,Yoshimi Kakinuma,Yoshiko Ishizuka-Katsura,Noboru Ohsawa,Takaho Terada,Mikako Shirouzu,Shigeyuki Yokoyama,So Iwata,Ichiro Yamato,Takeshi Murata,Takeshi Murata +15 more
TL;DR: These asymmetric structures represent the first high-resolution view of the rotational mechanism of V1-ATPase from the A3B3 and DF complexes and suggest a binding order in the right-handed rotational orientation in a cooperative manner.
Journal ArticleDOI
The crystal structure of plant-specific calcium-binding protein AtCBL2 in complex with the regulatory domain of AtCIPK14.
Mayuko Akaboshi,Hiroshi Hashimoto,Hanako Ishida,Shinya Saijo,Nozomu Koizumi,Mamoru Sato,Toshiyuki Shimizu +6 more
TL;DR: Structural comparison of the two structures shows that the recognition of CBL by CIPK is performed in a similar manner, but inherent interactions confer binding affinity and specificity.
Journal ArticleDOI
Crystal structure of the central axis DF complex of the prokaryotic V-ATPase.
Shinya Saijo,Satoshi Arai,K. M. Mozaffor Hossain,Ichiro Yamato,Kano Suzuki,Yoshimi Kakinuma,Yoshiko Ishizuka-Katsura,Noboru Ohsawa,Takaho Terada,Mikako Shirouzu,Shigeyuki Yokoyama,So Iwata,Takeshi Murata +12 more
TL;DR: The crystal structure of the DF complex of the prokaryotic V-ATPase of Enterococcus hirae, which is composed of the central axis D subunit and a membrane c ring that are connected by F and d subunits, is determined at 2.0-Å resolution.
Proceedings ArticleDOI
Upgrade of small angle x-ray scattering beamline BL-6A at the photon factory
Hideaki Takagi,Noriyuki Igarashi,T. Mori,Shinya Saijo,Hiromasa Ohta,Yasuko Nagatani,Takashi Kosuge,Nobutaka Shimizu +7 more
TL;DR: In this paper, a hybrid pixel detector PILATUS3 1M is installed for SAXS, and a pinhole equipped with a micro-ion chamber is available to realize a lower-background and higher-resolution of low angles.
Journal ArticleDOI
Mechanism of Catalytic Microtubule Depolymerization via KIF2-Tubulin Transitional Conformation
Tadayuki Ogawa,Shinya Saijo,Nobutaka Shimizu,Xuguang Jiang,Nobutaka Hirokawa,Nobutaka Hirokawa +5 more
TL;DR: To elucidate the catalytic mechanism, the transitional KIF2-tubulin complex during MT depolymerization was analyzed through multiple methods, including atomic force microscopy, size-exclusion chromatography, multi-angle light scattering, small-angle X-ray scattering, analytical ultracentrifugation, and mass spectrometry.