Shuobing Chen

TL;DR: It is found that the PrgJ-NAIP2-NLRC4 inflammasome is assembled into multisubunit disk-like structures through a unidirectional adenosine triphosphatase polymerization, primed with a single PrGJ-activated NAIP2 per disk.

TL;DR: Cryoelectron microscopy results reveal that the substrate-conducting channel in the core particle is transiently opened and accompanied by dynamic changes in structure of the particle, providing new insights into how the proteasome recognizes ubiquitylated substrates and translocates them through a channel and gate to degradation sites in thecore particle.

TL;DR: The results reveal that INCA caps caspase-1 filaments, thereby exerting potent inhibition with low-nanomolar Ki on casp enzyme-1CARD polymerization in vitro and inflammasome activation in cells.

TL;DR: It is shown that unsupervised GTM clustering improves classification accuracy by about 40% in the absence of input references for data with lower SNRs, and applications to several experimental datasets suggest that the algorithm can detect subtle structural differences among classes via a hierarchical clustering strategy.

TL;DR: The design and comprehensive characterization of a set of aromatic biosensors constructed using green fluorescence protein as the reporter and aromatics-responsive transcriptional regulators as the detectors are reported.