S
Silke Hoffmann
Researcher at University of Erlangen-Nuremberg
Publications - 4
Citations - 132
Silke Hoffmann is an academic researcher from University of Erlangen-Nuremberg. The author has contributed to research in topics: SH3 domain & SH2 domain. The author has an hindex of 3, co-authored 4 publications receiving 128 citations. Previous affiliations of Silke Hoffmann include University of Oxford.
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Journal ArticleDOI
Structural Investigation of the Binding of a Herpesviral Protein to the SH3 Domain of Tyrosine Kinase Lck
Kristian Schweimer,Silke Hoffmann,Finn Bauer,Ute Friedrich,Christian Kardinal,Stephan M. Feller,Brigitte Biesinger,Heinrich Sticht +7 more
TL;DR: Fluorescence spectroscopy shows that Tip binds to the SH3 domains of several Src kinases (Lck, Hck, Lyn, Src, Fyn, Yes), exhibiting the highest affinities for Lyn, Hock, and Lck.
Journal ArticleDOI
Binding, Domain Orientation, and Dynamics of the Lck SH3−SH2 Domain Pair and Comparison with Other Src-Family Kinases†
Gregor Hofmann,Kristian Schweimer,Anke Kiessling,Edith Hofinger,Finn Bauer,Silke Hoffmann,Paul Rösch,Iain D. Campbell,Jörn M. Werner,H. Sticht +9 more
TL;DR: Flexibility between SH2 and SH3 domains contributes to the adaptation of Src-family kinases to specific environments and distinct functions, according to the particular properties of the Lck SH3-SH2 linker which is shown to be extremely flexible.
Journal ArticleDOI
Characterization of Lck-binding elements in the herpesviral regulatory Tip protein.
TL;DR: NMR spectroscopy of isotopically labeled Tip revealed that the interaction with the LckSH3 domain is not restricted to the classical proline-rich motif, but also involves the C-terminally adjacent residues which pack into a hydrophobic pocket on the surface of the SH3 domain, thus playing a likely role in mediating binding specificity.
Protein Expression and Purification
TL;DR: The human hemofiltrate peptide HF6478, a putative serine proteinase inhibitor, which is part of the precursor protein LEKTI, was cloned, overexpressed, and purified as mentioned in this paper.