S
Simone Lemeer
Researcher at Utrecht University
Publications - 65
Citations - 7364
Simone Lemeer is an academic researcher from Utrecht University. The author has contributed to research in topics: Phosphorylation & Phosphopeptide. The author has an hindex of 34, co-authored 62 publications receiving 6389 citations. Previous affiliations of Simone Lemeer include Technische Universität München & Royal Netherlands Academy of Arts and Sciences.
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Journal ArticleDOI
Mass-spectrometry-based draft of the human proteome
Mathias Wilhelm,Judith Schlegl,Hannes Hahne,Amin Moghaddas Gholami,Marcus Lieberenz,Mikhail M. Savitski,Emanuel Ziegler,Lars Butzmann,Siegfried Gessulat,Harald Marx,Toby Mathieson,Simone Lemeer,Karsten Schnatbaum,Ulf Reimer,Holger Wenschuh,Martin Mollenhauer,Julia Slotta-Huspenina,Joos-Hendrik Boese,Marcus Bantscheff,Anja Gerstmair,Franz Faerber,Bernhard Kuster +21 more
TL;DR: A mass-spectrometry-based draft of the human proteome and a public, high-performance, in-memory database for real-time analysis of terabytes of big data, called ProteomicsDB are presented, which enables navigation of proteomes, provides biological insight and fosters the development of proteomic technology.
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Multiplex peptide stable isotope dimethyl labeling for quantitative proteomics.
Paul J. Boersema,Reinout Raijmakers,Simone Lemeer,Simone Lemeer,Shabaz Mohammed,Albert J. R. Heck +5 more
TL;DR: In-solution, online and on-column protocols for stable isotope dimethyl labeling of sample amounts ranging from sub-micrograms to milligrams are described, which uses inexpensive reagents and is applicable to virtually any sample.
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Quantitative mass spectrometry in proteomics: critical review update from 2007 to the present
TL;DR: The notion that mass spectrometry has the potential to eventually retire the western blot is no longer in the realm of science fiction and major technical and conceptual developments since 2007 are focused on.
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Confident Phosphorylation Site Localization Using the Mascot Delta Score
Mikhail M. Savitski,Simone Lemeer,Markus Boesche,Manja Lang,Toby Mathieson,Marcus Bantscheff,Bernhard Kuster,Bernhard Kuster +7 more
TL;DR: The Mascot Delta Score is validated as a simple method that achieves similar sensitivity and specificity for phosphosite localization as the published Ascore and it is shown that the ability to call sites correctly increases with increasing distance of two candidate sites within a peptide sequence.
Journal ArticleDOI
DMSO enhances electrospray response, boosting sensitivity of proteomic experiments
Hannes Hahne,Fiona Pachl,Benjamin Ruprecht,Stefan Maier,Susan Klaeger,Dominic Helm,Guillaume Médard,Matthias Wilm,Simone Lemeer,Bernhard Kuster,Bernhard Kuster +10 more
TL;DR: It is reported that low percentages of dimethylsulfoxide (DMSO) in liquid chromatography solvents lead to a strong enhancement of electrospray ionization of peptides, improving the sensitivity of protein identification in bottom-up proteomics by up to tenfold.