Sohini Sanyal

TL;DR: Evidence is provided that the stevor and Pfmc‐2TM gene families play a role in P. falciparum antigenic variation, and chromosomal telomeric deletions are common in clonal lines and result in a spectrum of deletion genotypes.

TL;DR: Evidence is presented that the Stevor and Pfmc-2TM families are exported to the erythrocyte membrane, thus supporting the hypothesis that host immune pressure drives antigenic diversity within the loop, and an examination of multiple P.falciparum isolates demonstrates that the hypervariable loop within Stevor et al. possesses sequence diversity across isolate boundaries.

TL;DR: It is shown that stevor expression impacts deformability of the erythrocyte membrane, which may facilitate parasite sequestration in deep tissue vasculature.

TL;DR: In the course of producing transgenic lines from the original NF54 parasite isolate, cloned and characterised a parasite line, termed E5, which is closely related to but distinct from 3D7, the parasite used for the P. falciparum genome nucleotide sequencing project, and identified examples of frequent recombination events within these gene families, including an example of a duplicative transposition.

TL;DR: The materials and methods required to performreal-time RT-PCR, including RNA purification, cDNA synthesis, and real-time PCR analysis of cDNA samples are described.