Srujan Kumar Dondapati

TL;DR: Gold nanostars provide high sensitivity for single nanoparticle label-free biosensing and Streptavidin molecules are detected upon binding to individual, biotin-modified gold nanostar molecules by spectral shifts in the plasmon resonances.

TL;DR: Current state‐of‐the‐art methodologies of membrane protein synthesis in biomimetic‐supported systems are summarized and cell‐free systems utilizing the advantages of biological membranes are highlighted.

TL;DR: Optical spectroscopy of voltage-clamped single gold nanoparticles reveals evidence that anion adsorption starts at positive potentials above the point of zero charge, causing severe but reversible plasmon damping in combination with a spectral red shift exceeding the linear double layer charging effect.

TL;DR: This study demonstrated the ability to produce high yields of various protein types including membrane proteins and single chain variable fragments (scFv) in a continuous exchange cell-free (CECF) system based on CHO cell lysate that contains endogenous microsomal structures and proved biological activity of synthesized proteins using various analysis technologies.

TL;DR: This article discusses some recent progress in cell-free systems derived from Chinese hamster ovary and Sf 21 lysates containing endogenous translocationally active microsomes for the synthesis of membrane proteins.