Stefano Bettati

TL;DR: The relevance of recent genomic analysis of PLP-dependent enzymes for the selection of drug targets is discussed, and enzymes that have been recently characterized and proposed as drug targets are reported.

TL;DR: The model is consistent with the results of solution, gel, and single crystal oxygen binding studies, but underestimates the population of doubly-liganded molecules determined in low-temperature electrophoresis experiments.

TL;DR: In this article, the T state of hemoglobin in silica gels was determined to have a reduced affinity compared with solution, with no Bohr effect and with no influence of other allosteric effectors.

TL;DR: Comparing various allosteric models to explain cooperative oxygen binding to hemoglobin shows the preponderance of experimental evidence favors the TTS model which postulates an equilibrium between high (r)‐ and low (t)‐affinity tertiary conformations that are present in both the T and R quaternary structures.

TL;DR: In this paper, the authors investigated the kinetic properties of unstable conformations transiently trapped by encapsulation in silica gels and found that after nanosecond photodissociation of carbon monoxide, a large fraction of the subunits of the T quaternary structure had kinetic properties almost identical to those of sub units of the R quaternaries.