Steve Lefever

TL;DR: Single mismatches located >5 bp from the 3' end have a moderate effect on qPCR amplification and can be tolerated, and this finding, together with the concentration independence for single mismatches and the complete blocking of the PCR reaction for ≥4 mismatches, can help to chart mismatch behavior inqPCR reactions and increase the rate of successful primer design for sequences with a high SNP density or for homologous regions of sequence.

TL;DR: MiRBase Tracker is developed, an easy-to-use online database that keeps track of all historical and current miRNA annotation present in the miRBase database, and is expected to increase the transparency and annotation accuracy in the field of miRNA research.

TL;DR: Evidence that murine neural crest progenitor cells can give rise to neuroblastoma upon transformation with MYCN or ALKF1174L is presented and indicates that neuroblastomas arise as their malignant progeny.

TL;DR: This work presents an innovative approach to elucidate tissue-specific miRNA functions that goes beyond mi RNA target prediction and expression correlation, based on a multi-level integration of corresponding miRNA and mRNA gene expression levels, miRNA target prediction, transcription factor target predictions and mechanistic models of gene network regulation.

TL;DR: It is demonstrated that CHAF1A restricts neural crest differentiation and contributes to the pathogenesis of high-risk neuroblastoma and loss of function was sufficient to drive neuronal differentiation in vitro and in vivo.