S
Sven Sagasser
Researcher at Ludwig Institute for Cancer Research
Publications - 3
Citations - 2890
Sven Sagasser is an academic researcher from Ludwig Institute for Cancer Research. The author has contributed to research in topics: Gene & Illumina dye sequencing. The author has an hindex of 3, co-authored 3 publications receiving 2110 citations.
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Full-length RNA-seq from single cells using Smart-seq2
Simone Picelli,Omid R. Faridani,Åsa K. Björklund,Gösta Winberg,Sven Sagasser,Rickard Sandberg +5 more
TL;DR: In this article, the authors presented a detailed protocol for Smart-seq2 that allows the generation of full-length cDNA and sequencing libraries by using standard reagents, and the entire protocol takes ∼2 d from cell picking to having a final library ready for sequencing; sequencing will require an additional 1-3 d depending on the strategy and sequencer.
Journal ArticleDOI
Exploring parasite heterogeneity using single-cell RNA-seq reveals a gene signature among sexual stage Plasmodium falciparum parasites
Mtakai Ngara,Mia Palmkvist,Sven Sagasser,Daisy Hjelmqvist,Åsa K. Björklund,Mats Wahlgren,Johan Ankarklev,Johan Ankarklev,Johan Ankarklev,Rickard Sandberg,Rickard Sandberg +10 more
TL;DR: A capillary‐based platform for cell isolation with single‐cell RNA‐sequencing to transcriptionally profile 165 single infected red blood cells during the intra‐erythrocytic developmental cycle (IDC) and a gene signature is uncovered that can successfully discriminate between developing asexual and sexual stage parasites at cellular resolution.
Journal ArticleDOI
Comparative transcriptomics reveal developmental turning points during embryogenesis of a hemimetabolous insect, the damselfly Ischnura elegans
Sabrina Simon,Sabrina Simon,Sven Sagasser,Edoardo Saccenti,Mercer R. Brugler,Mercer R. Brugler,M. Eric Schranz,Heike Hadrys,Heike Hadrys,Heike Hadrys,George Amato,Rob DeSalle +11 more
TL;DR: This work reconstructed the first comparative transcriptome covering the daily embryonic developmental progression of the blue-tailed damselfly Ischnura elegans (Odonata), an ancient hemimetabolous representative and identified a “core” set of 6,794 transcripts which are mainly involved in anatomical structure development and cellular nitrogen compound metabolic processes.