Tamara Hudnik-Plevnik

TL;DR: Using the reduced CO difference spectra, the enzyme cytochrome P-450 is located in the membrane fraction sedimenting at 105.000 g and the hydroxylating system in Rhizopus nigricans was found in post-mitochondrial supernatant.

TL;DR: It is concluded that the 11α-hydroxylation of progesterone in R. nigricans and R. arrhizus requires the induction, both, of cytochrome P-450 and of NADPH cy tochrome-C reductase.

TL;DR: Results of experiments presented can be regarded as the first strong indication that the biological role of P45011α induction in R. nigricans is in removal of steroids which are toxic for the mycelium.

TL;DR: Study of the induction of progesterone hydroxylating enzymes in Rhizopus nigricans revealed that the induction proceeds much faster in the buffer solution than in the growth medium, indicating that the progester one hydroxolating enzymes are inducible in this organism.

TL;DR: It is shown that the presence of all three components was necessary for the reconstitution of the active electron transport chain in the eucaryotic filamentous fungus Rhizopus nigricans.