Journal ArticleDOI
Resolution and reconstitution of cytochrome P-450 containing steroid hydroxylation system of Rhizopus nigricans.
TLDR
It is shown that the presence of all three components was necessary for the reconstitution of the active electron transport chain in the eucaryotic filamentous fungus Rhizopus nigricans.About:
This article is published in Journal of Steroid Biochemistry.The article was published on 1987-04-01. It has received 21 citations till now. The article focuses on the topics: Rhizopus nigricans & Cytochrome.read more
Citations
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Journal ArticleDOI
Microbial conversion of steroid compounds: recent developments
Pedro Fernandes,Pedro Fernandes,A. Cruz,B. Angelova,B. Angelova,Helena M. Pinheiro,Joaquim M. S. Cabral +6 more
TL;DR: An overview of recent developments on the use of microorganisms for steroid production is presented in order to increase the efficiency of the existing processes as well as to identify new potentially useful bioconversions.
Journal ArticleDOI
Current trends in microbial steroid biotransformation.
Shashi B. Mahato,Ipsita Majumdar +1 more
TL;DR: Various steroid substrates, their metabolites and the micro-organisms used for the transformations are compiled covering the literature for the period late 1987-mid 1992.
Book ChapterDOI
The biodiversity of microbial cytochromes P450
TL;DR: This review seeks to describe the historical development of CYP studies and to highlight the importance of the genes and proteins in pathways of secondary metabolism that include CYPs in oxidative tailoring of antibiotics, sometimes influencing potency as bioactive compounds.
Journal ArticleDOI
Microbial transformations of steroids-XI. Progesterone transformation by Streptomyces roseochromogenes–purification and characterisation of the 16α-hydroxylase system
TL;DR: Yield data show that in the reconstituted natural pathway, progesterone 16alpha-hydroxylase P450 supports multiple rounds of hydroxylation in contrast to a likely single oxygenation by a minority of P450s in the peroxide shunt pathway.
Journal ArticleDOI
Microbial transformations of steroids—X. Cytochromes P-450 11α-hydroxylase and C17–C20 lyase and a 1-ene dehydrogenase transform steroids in Nectria haematococca
TL;DR: Nectria haematococca contains four enzymes that metabolise exogenous steroids that act sequentially on progesterone producing firstly, by side-chain cleavage, the C19 steroid androstenedione (C17-C20 lyase), and then, in a subsequent set of transformations, 11 alpha-hydroxylated derivatives (11 alpha-Hydroxylase).
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
The Carbon Monoxide-binding Pigment of Liver Microsomes I. EVIDENCE FOR ITS HEMOPROTEIN NATURE
Tsuneo Omura,Ryo Sato +1 more
TL;DR: The present paper gives a detailed account of the investigations on rabbit liver microsomes and crude microsomal digests, which have led to postulate the hemoprotein nature of the pigment.
Book ChapterDOI
Purification and properties of NADPH-cytochrome P-450 reductase.
TL;DR: The procedure reported in the chapter describes the purification to apparent homogeneity of NADPH cytochrome P-450 reductase by solubilization with Renex 690 and affinity chromatography.
Journal ArticleDOI
Microbiological oxygenation of steroids at carbon 11
D. H. Peterson,H. C. Murray +1 more
Journal ArticleDOI
Properties of a Yeast Cytochrome P‐450‐Containing Enzyme System which Catalyzes the Hydroxylation of Fatty Acids, Alkanes, and Drugs
TL;DR: A yeast lysophosphati-dylethanolamine fraction was shown to be most effective as judged by laurate hydroxylation, and the yeast cytochrome P-450 is not readily autoxidizable, as shown by experiments in which it was reduced extensively by NADPH in the presence of phospholipid and the reductase under aerobic conditions.