Showing papers by "Thomas Maciag published in 1994"
TL;DR: Analysis of the effect of interleukin-1 alpha on the expression of Cox-2 in a human cell line and post-transcriptional mechanisms are suggested to be important in the sustained induction of thecox-2 mRNA suggest that IL-1alpha may increase the stability of the Cox- 2 transcript.
427 citations
TL;DR: It is proposed that the association of c-Src with activated FGF receptors may be responsible for the tyrosine phosphorylation of cortactin during the mid to late G1 phase of the cell cycle.
169 citations
TL;DR: The results suggest that the FGFR-1 may be translocated near the nucleus upon interaction with its ligand during the entire G1 period of the NIH 3T3 cell cycle as a structurally intact and functional tyrosine kinase that may be accessible to perinuclear polypeptides as a regulatory enzyme.
77 citations
TL;DR: It is demonstrated that the elevated level of IL-1 alpha mRNA in the senescent population of strain H3605 may be regulated by mRNA stability; however, this mechanism does not apply to all the HUVEC strains examined in this study.
Abstract: Human umbilical vein endothelial cell (HUVEC) senescence in vitro is characterized by the loss of proliferative potential and an increase in cell size. Because HUVEC senescence in one strain (H101) has been characterized by the increase in the steady-state mRNA level for the signal-peptideless cytokine, interleukin (IL) 1 alpha, we have examined young and senescent populations of five additional HUVEC strains (H3605, H103, H928, H929, and H930) to determine whether the elevated levels of IL-1 alpha mRNA could be observed in all HUVEC strains. Consistent with the data from strain H101, strains H3605 and H930 also exhibited a low steady-state level of the IL-1 alpha mRNA in young populations compared to elevated levels of IL-1 alpha mRNA in the senescent populations. However, three strains (H103, H928, and H929) did not exhibit reduced levels of IL-1 alpha mRNA in the young populations, and interestingly, strain H928, at times, expressed relatively high IL-1 alpha mRNA levels in the young populations. In addition, expression of the steady-state level of plasminogen activator inhibitor 1 and cyclooxygenase 2 was elevated in senescent populations of all HUVEC strains examined, whereas young populations exhibited a low level of expression for these genes regardless of the IL-1 alpha mRNA level. Further, the level of the IL-1 alpha polypeptide was elevated in senescent HUVEC populations relative to young populations that expressed either a high or low level of the IL-1 alpha mRNA. We have also demonstrated that the elevated level of IL-1 alpha mRNA in the senescent population of strain H3605 may be regulated by mRNA stability; however, this mechanism does not apply to all the HUVEC strains examined in this study. Thus, we suggest that while mRNA levels of the IL-1-response genes for plasminogen activator inhibitor 1 and cyclooxygenase 2 are appropriate markers for HUVEC senescence, HUVEC strain-specific post-transcriptional mechanisms may exist to regulate the function of IL-1 alpha as a modifier of HUVEC senescence in vitro.
71 citations
TL;DR: It is suggested that the reduced mitogenic activity observed in preparations of the FGF-1(28-154) deletion mutant may be the result of structural instability and fluorescence spectroscopy cannot be used to predict FGF -1 stability.
30 citations
TL;DR: The mechanisms used by the FGFs to regulate cell division are reviewed, including the ability to bind the glycosaminoglycan heparin, a property that has led to the initiation of the alternative FGF nomenclature as theHeparin-binding growth factors.
Abstract: Publisher Summary The fibroblast growth factors (FGFs) are a multitalented family of polypeptides with a broad biological base. The FGFs are mitogens, chemoattractants, and differentiation factors for a wide variety of diverse vertebrate cell types in vitro and are known to be potent angiogenic and neurotrophic factors in vivo. The FGFs are also recognized as important contributors to a variety of pathophysiological situations in vivo, including rheumatoid arthritis, solid tumor growth, and atherogenesis. This chapter reviews the mechanisms used by the FGFs to regulate cell division. The FGF gene family is currently comprised of eight structurally related members, including two prototypes, acidic FGF, basic FGF, and six additional members, five of which are oncogenes. A feature that discriminates among the FGF prototypes and the FGF oncogenes is the absence of a classical signal peptide sequence within the FGF prototypes to direct their secretion using conventional secretory pathways. A common feature that the FGF family members share is the ability to bind the glycosaminoglycan heparin, a property that has led to the initiation of the alternative FGF nomenclature as the heparin-binding growth factors.
16 citations