Showing papers in "Biochemical and Biophysical Research Communications in 1994"

TL;DR: Data implicate Ginkgo biloba extract EGb 761 as a potential therapeutic agent in conditions of altered production of nitric oxide in in vitro acellular systems.

TL;DR: The chemical structure of rottlerin might serve as a basis for the development of novel inhibitors with improved selectivity for a distinct PKC isoenzyme, such as PKC delta, or for CaM-kinase III.

TL;DR: It is suggested that ETB receptors play an important role in the clearance of ET-1, as about 80% of bolus-injected [125I]ET-1 was retained by the lungs after one passage.

TL;DR: Active production of adrenomedullin in cultured vascular endothelial cells (ECs) is demonstrated and AM secreted from ECs is deduced to act directly on VSMCs, regulating vascular tone.

TL;DR: It is reported that Aβ(25-35) is a potent lipoperoxidation initiator, as inferred from peptide-mediated reduction of nitroxyl stearate spin labels bound to rodent neocortical synaptosomal membranes, a "molecular shrapnel" model of neuronal membrane damage in Alzheimer′s disease.

TL;DR: Treatment of cartilage fragments with L-NG-monomethylarginine (L-NMA), a competitive inhibitor of NO synthase, both inhibited NO synthesis in response to IL-1 and CAF and restored proteoglycan synthesis, suggesting that antagonists of NO production may promote cartilage matrix synthesis and thus have potential as chondroprotective or chondrogenic agents.

TL;DR: Production and secretion of adrenomedullin from cultured vascular smooth muscle cells (VSMCs) are demonstrated and AM is deduced to function as an autocrine or paracrine regulator in vascular cell communication.

TL;DR: L86-8275 directly inhibits immunoprecipitated Cdc2 kinase activity from G2/M synchronized MDA-MB-468 breast carcinoma cells and is at least 250-fold more potent than either quercetin or genistein.

TL;DR: It is demonstrated that cytokines, such as those released by mononuclear cells, can induce lung epithelial iNOS expression and NO release, and that this is attenuated by dexamethasone.

TL;DR: A newly identified human peptide, adrenomedullin (AM) increased cAMP levels with dose and time dependencies in a manner similar to that of human calcitonin gene-related peptide (CGRP) in rat vascular smooth muscle cells (VSMC).

TL;DR: The ability of conjugated linoleic acid to prevent endotoxin-induced growth suppression and Splenocyte blastogenesis was increased by conjugations, and anorexia was prevented from endotoxin injection.

TL;DR: Structural-activity studies indicated that amino- and carboxyl-terminal portions of alpha-MSH appear to be key determinants in the activation of mMC5R whereas the melanocortin core heptapeptide sequence is devoid of pharmacological activity.

TL;DR: Findings reveal a direct action of NO on the mitochondrial respiratory chain and suggest that NO exerts some of its physiological and pathological effects by deenergizing mitochondria.

TL;DR: The results suggest that VAS/VEGF could be an important regulator of osteoblastic differentiation, and the maximal effects reach levels comparable to that obtained with bone morphogenetic protein 2 (BMP-2), although the VAS-VEGF concentrations required are at least 100 fold lower.

TL;DR: The isolation of the entire PGHS-2 gene and its 5'-flanking region from a human bacteriophage P1 genomic library showed that the first 460 nucleotides of 5'-Flanking region efficiently drove transcription of the luciferase reporter gene in human umbilical vein endothelial cells upon stimulation with phorbor ester.

TL;DR: Results show that nitric oxide interacts with and destroys zinc-sulfur clusters in proteins and inhibits DNA binding activity of the yeast transcription factor LAC9 containing a zinc finger like DNA binding domain.

TL;DR: In this article, the authors demonstrate the DNA binding activity of nuclear factor kappa B (NF kappaB), a member of the Rel Family of transcription factors, and nuclear factor activator protein-1 (AP-1) following exposure of endothelial cells to unidirectional shear stress in laminar flow.

TL;DR: Structural-activity analysis suggests that gallic acid induces apoptosis in HL-60RG cells, depending on its distinctive feature derived from the structure but not on its antioxidative activity.

TL;DR: The results suggest that in airways the initial response to oxidative stress may be to induce NF k B-responsive genes, such as iNOS, which may play an important role in defending the airway against oxidative stress.

TL;DR: DDP-induced CCLA was completely inhibited by the addition of an O2- scavenger, superoxide dismutase (SOD), or an antioxidant, ascorbic acid, and heat-inactivated SOD did not affect CCLA.

TL;DR: Northern blot analysis using RNA from multiple tissues indicated that ROR gamma is expressed in several tissues but is most highly expressed in skeletal muscle.

TL;DR: All 5 human SSTRs are functionally coupled to inhibition of adenylyl cyclase in CHO-K1 cells via pertussis toxin sensitive G proteins, demonstrating their binding affinities for the individual hSSTR subtypes.

TL;DR: The human endothelium-derived relaxing factor (EDRF)/nitric oxide (NO) is synthesized from L-Arginine by the endothelial, constitutive, NO synthase gene and its structure is determined.

TL;DR: Low levels of mitochondrial transcription factor A (mtTFA) were found in muscle fibers with mitochondrial DNA (mtDNA) depletion in a child with fatal mitochondrial myopathy and also in a human cell line lacking mtDNA.

TL;DR: F2Pmp may be a generally useful tool in the preparation of selective, high affinity PTP inhibitors, and induces a three orders of magnitude enhancement in affinity relative to Pmp, resulting in an exceptionally potent peptide-based PTP inhibitor.

TL;DR: Findings suggest that, in addition to bone formation, OP-1 could have other important regulatory roles in human embryogenesis with high binding affinity to a basement membrane component.

TL;DR: Two proteins produced from the nonstructural region 5A (NS5A), which has the potential to produce a 49 kDa protein, are shown to be phosphorylated at the serine residues and the hyper-phosphorylation of p58 was shown to depend on the presence of NS4A, another hepatitis C virus protein.

TL;DR: A significant increase in NOS activity in microvessels isolated from Alzheimer brains is indicated and elevated vascular production of NO, a potentially neurotoxic mediator in the CNS, may contribute to the susceptibility of neurons to injury and cell death in Alzheimer's disease.

TL;DR: The cloning, pharmacological characterization and expression of the rat fifth melanocortin receptor (MC5) is reported, starting from the dopamine D3 receptor sequence to screen a genomic DNA library.

TL;DR: Results show that lung epithelial cells can release NO, a process which can be inhibited by dexamethasone.