T
Thomas Walzthoeni
Researcher at ETH Zurich
Publications - 18
Citations - 4027
Thomas Walzthoeni is an academic researcher from ETH Zurich. The author has contributed to research in topics: Protein structure & Mass spectrometry. The author has an hindex of 16, co-authored 16 publications receiving 3722 citations. Previous affiliations of Thomas Walzthoeni include University of Innsbruck & University of Zurich.
Papers
More filters
Journal ArticleDOI
Molecular architecture of the 26S proteasome holocomplex determined by an integrative approach
Keren Lasker,Friedrich Förster,Stefan Bohn,Thomas Walzthoeni,Elizabeth Villa,Pia Unverdorben,Florian Beck,Ruedi Aebersold,Andrej Sali,Wolfgang Baumeister +9 more
TL;DR: In this paper, the molecular structure of the 26S holocomplex was determined by an integrative approach based on data from cryoelectron microscopy, X-ray crystallography, residue-specific chemical cross-linking, and several proteomics techniques.
Journal ArticleDOI
Probing Native Protein Structures by Chemical Cross-linking, Mass Spectrometry, and Bioinformatics
Alexander Leitner,Thomas Walzthoeni,Thomas Walzthoeni,Abdullah Kahraman,Franz Herzog,Oliver Rinner,Martin Beck,Ruedi Aebersold,Ruedi Aebersold +8 more
TL;DR: In this article, the authors discuss the critical steps of chemical cross-linking and its implications for (structural) biology: reagent design and cross-link protocols, separation and mass spectrometric analysis of cross-linked samples, dedicated software for data analysis, and the use of crosslink data for computational modeling.
Journal ArticleDOI
Identification of cross-linked peptides from large sequence databases
Oliver Rinner,Jan Seebacher,Thomas Walzthoeni,Thomas Walzthoeni,Lukas N. Mueller,Martin Beck,Alexander Schmidt,Alexander Schmidt,Markus Mueller,Ruedi Aebersold,Ruedi Aebersold,Ruedi Aebersold +11 more
TL;DR: It is shown that xQuest can identify cross-linked peptides from a total Escherichia coli lysate with an unrestricted database search and reduces the search space by an upstream candidate-peptide search before the recombination step.
Probing Native Protein Structures by Chemical Cross-linking, Mass Spectrometry, and Bioinformatics
Alexander Leitner,Thomas Walzthoeni,Thomas Walzthoeni,Abdullah Kahraman,Franz Herzog,Oliver Rinner,Martin Beck,Ruedi Aebersold,Ruedi Aebersold +8 more
TL;DR: The critical steps of chemical cross-linking and its implications for (structural) biology are discussed: reagent design and cross- linking protocols, separation and mass spectrometric analysis of cross-linked samples, dedicated software for data analysis, and the use ofCross-l linking data for computational modeling.
Journal ArticleDOI
Structural Probing of a Protein Phosphatase 2A Network by Chemical Cross-Linking and Mass Spectrometry
Franz Herzog,Abdullah Kahraman,Daniel Boehringer,Raymond H. Mak,Andreas Bracher,Thomas Walzthoeni,Alexander Leitner,Martin Beck,Franz-Ulrich Hartl,Nenad Ban,Lars Malmström,Ruedi Aebersold +11 more
TL;DR: This study establishes XL-MS as an integral part of hybrid structural biology approaches for the analysis of endogenous protein complexes by gaining distance restraints on a modular interaction network of protein complexes affinity-purified from human cells by applying an adaptedXL-MS protocol.