Tianxiang Tu

TL;DR: This work developed a directional screening system in human cells to evolve the deaminase component of the ABE, and identified three high-activity NG-ABEmax variants, and created a new, consolidated variant which exhibited superior editing activity both inhuman cells and in mouse disease models.

TL;DR: The results revealed that BEs triggered more off-target effects in the genome than on plasmids including genomic indels (insertions and deletions), and it was found that ABEs (adenine base editors) had better fidelity than CBEs (cytosine base Editors).

TL;DR: In this paper, a high-throughput human cells-based reporter system that can be harnessed for quickly measuring editing activity of Cytosine base editor (CBE) was reported.

TL;DR: In this article , NG-ABE9e exhibits robust and precise base-editing activity in human cells, with more than 7-fold bystander editing reduction at some sites.

TL;DR: It was found that SpCas9 and SaCas9, the most two commonly used Cas9 enzymes, possessed a similar ability to induce HDR and with the increasing amount of coding plasmids or additional nuclear localization sequences (NLS), FnCpf1 could improve the HDR efficiency.