Xiujuan Lv

TL;DR: This study describes a directional screening system and provides a general strategy to rapidly evolve other desired CRISPR-Cas9 traits besides enhanced fidelity, to expand the utility of theCRISPR toolkit.

TL;DR: BMs display excellent biocompatibility and potential for use in the treatment of ocular diseases as well as cytotoxicity and genotoxicity studies in human retinal pigment epithelium cells.

TL;DR: FnCpf1, a powerful genome-editing tool for multiple genomic targets, can be harnessed for effective manipulation of the human genome by utilizing a single customized crRNA array to simultaneously target multiple genes in human cells.

TL;DR: The SaCas9-RNP displayed efficient genome editing activities of enhanced green fluorescent protein (EGFP) coding gene as well as three endogenous genes (OPA1, RS1, and VEGFA), which could facilitate genome-editing-based therapy.

TL;DR: This work developed a directional screening system in human cells to evolve the deaminase component of the ABE, and identified three high-activity NG-ABEmax variants, and created a new, consolidated variant which exhibited superior editing activity both inhuman cells and in mouse disease models.