Showing papers by "Tuhin Subhra Santra published in 2023"
TL;DR: In this paper , fluorescent polymeric nano-thermometers (FPNTs) were used to measure the intratumor temperature in co-cultured 3D tumor spheroids.
Abstract: Temperature governs the reactivity of a wide range of biomolecules in the cellular environment dynamically. The complex cellular pathways and molecules in solid tumors substantially produce temperature gradients in the tumor microenvironment (TME). Hence, visualization of these temperature gradients at the cellular level would give physiologically relevant spatio-temporal information about solid tumors. This study used fluorescent polymeric nano-thermometers (FPNTs) to measure the intratumor temperature in co-cultured 3D tumor spheroids. A temperature-sensitive rhodamine-B dye and Pluronic F-127 were conjugated through hydrophobic and hydrophobic interactions and then cross-linked with urea-paraformaldehyde resins to form the FPNTs. The characterization results exhibit monodisperse nanoparticles (166 ± 10 nm) with persistent fluorescence. The FPNTs exhibit a linear response with a wide temperature sensing range (25-100 °C) and are stable toward pH, ionic strength, and oxidative stress. FPNTs were utilized to monitor the temperature gradient in co-cultured 3D tumor spheroids and the temperature difference between the core (34.9 °C) and the periphery (37.8 °C) was 2.9 °C. This investigation demonstrates that the FPNTs have great stability, biocompatibility, and high intensity in a biological medium. The usage of FPNTs as a multifunctional adjuvant may demonstrate the dynamics of the TME and they may be suitable candidates to examine thermoregulation in tumor spheroids.
2 citations
TL;DR: The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online as discussed by the authors , which is calculated using the Alt-metric attention score and how the score is calculated.
Abstract: ADVERTISEMENT RETURN TO ISSUEReviewNEXTRecent Advances of Biosensor-Integrated Organ-on-a-Chip Technologies for Diagnostics and TherapeuticsAshwini ShindeAshwini ShindeDepartment of Engineering Design, Indian Institute of Technology Madras, Chennai 600036, IndiaMore by Ashwini ShindeView Biography, Kavitha IllathKavitha IllathDepartment of Engineering Design, Indian Institute of Technology Madras, Chennai 600036, IndiaMore by Kavitha IllathView Biography, Uvanesh KasiviswanathanUvanesh KasiviswanathanDepartment of Engineering Design, Indian Institute of Technology Madras, Chennai 600036, IndiaMore by Uvanesh KasiviswanathanView Biographyhttps://orcid.org/0000-0003-3535-2244, Shalini NagabooshanamShalini NagabooshanamDepartment of Engineering Design, Indian Institute of Technology Madras, Chennai 600036, IndiaMore by Shalini NagabooshanamView Biographyhttps://orcid.org/0000-0003-4856-6815, Pallavi GuptaPallavi GuptaDepartment of Engineering Design, Indian Institute of Technology Madras, Chennai 600036, IndiaMore by Pallavi GuptaView Biography, Koyel DeyKoyel DeyDepartment of Engineering Design, Indian Institute of Technology Madras, Chennai 600036, IndiaMore by Koyel DeyView Biography, Pulasta ChakrabartyPulasta ChakrabartyDepartment of Engineering Design, Indian Institute of Technology Madras, Chennai 600036, IndiaMore by Pulasta ChakrabartyView Biography, Moeto NagaiMoeto NagaiDepartment of Mechanical Engineering, Toyohashi University of Technology, Toyohashi 441-8580, JapanMore by Moeto NagaiView Biography, Suresh RaoSuresh RaoDepartment of Engineering Design, Indian Institute of Technology Madras, Chennai 600036, IndiaMore by Suresh RaoView Biography, Srabani Kar*Srabani KarDepartment of Physics, Indian Institute of Science Education and Research (IISER), Tirupati, Andhra Pradesh 517507, India*[email protected]More by Srabani KarView Biography, and Tuhin Subhra Santra*Tuhin Subhra SantraDepartment of Engineering Design, Indian Institute of Technology Madras, Chennai 600036, India*[email protected]; [email protected]More by Tuhin Subhra SantraView Biographyhttps://orcid.org/0000-0002-9403-2155Cite this: Anal. Chem. 2023, 95, 6, 3121–3146Publication Date (Web):January 30, 2023Publication History Received12 November 2022Published online30 January 2023Published inissue 14 February 2023https://doi.org/10.1021/acs.analchem.2c05036Copyright © 2023 American Chemical SocietyRIGHTS & PERMISSIONSArticle Views1140Altmetric-Citations1LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit Read OnlinePDF (3 MB) Get e-AlertsSUBJECTS:Biotechnology,Cells,Electrical properties,Electrodes,Sensors Get e-Alerts
2 citations
TL;DR: In this paper , a triple-layered structure is developed in a 3D graphene foams (GF) matrix to closely mimic native tissue structures of the periodontium of the teeth.
Abstract: Physiological bioengineering of multilayered tissues requires an optimized geometric organization with comparable biomechanics. Currently, polymer-reinforced three-dimensional (3D) graphene foams (GFs) are gaining interest in tissue engineering due to their unique morphology, biocompatibility, and similarity to extracellular matrixes. However, the homogeneous reinforcement of single polymers throughout a GF matrix does not provide tissue-level organization. Therefore, a triple-layered structure is developed in a GF matrix to closely mimic native tissue structures of the periodontium of the teeth. The scaffold aims to overcome the issue of layer separation, which generally occurs in multilayered structures due to the poor integration of various layers. The 3D GF matrix was reinforced with a polycaprolactone (PCL), polyvinyl alcohol (PVA), and PCL-hydroxyapatite (HA) mixture, added sequentially, via spin coating, vacuum, and hot air drying. Later, PVA was dissolved to create a middle layer, mimicking the periodontal fibers, while the layers present on either side resembled cementum and alveolar bone, respectively. Scanning electron microscopy and micro-computed tomography revealed the structure of the scaffold with internal differential porosities. The nanoindentation and tensile testing demonstrated the closeness of mechanical properties to that of native tissues. The biocompatibility was assessed by the MTT assay with MG63 cells (human osteosarcoma cells) exhibiting high adhesion and proliferation rate inside the 3D architecture. Summing up, this scaffold has the potential for enhancing the regeneration of various multilayered tissues.
1 citations
TL;DR: In this paper , the authors reported highly efficient, uniform parallel intracellular delivery of small to very large biomolecules into diverse cell types using a titanium micro-ring (TMR) device activated by infrared (IR) light pulse.
Abstract: Uniform transfection of biomolecules into live cells with high delivery efficiency and cell viability is an immensely important area of biological research and has many biomedical applications. In the present study, we report highly efficient, uniform parallel intracellular delivery of small to very large biomolecules into diverse cell types using a titanium micro-ring (TMR) device activated by infrared (IR) light pulse. A TMR array device (2 cm × 2 cm) consists of a 10 μm outer diameter and 3 μm inner diameter for each micro-ring, and 10 μm interspacing between two micro-rings. Upon IR (1050 nm) pulse laser irradiation on the TMR device, photothermal cavitation bubbles are generated, disrupting the cell plasma membrane, and biomolecules are gently delivered into the cells by a simple diffusion process. This TMR device successfully delivered diverse types of small to very large biomolecules such as propidium iodide (PI; 668.4 Da) dye, dextran (3 kDa), small interfering RNA (13.3 kDa), enhanced green fluorescent protein expression plasmid DNA (6.2 kb), and β-galactosidase enzyme (465 kDa) into human cervical (SiHa), mouse fibroblast (L929), and mouse neural crest-derived (N2a) cancer cells. For smaller molecules (PI dye), delivery efficiency and cell viability were achieved at ∼96% and ∼97%, respectively, with a laser fluence of 21 mJ cm-2 for 250 pulses. In contrast, ∼85% transfection efficiency and ∼90% cell viability were achieved for plasmid DNA with 45 mJ cm-2 laser fluence for 250 pulses in SiHa cells. Moreover, the intracellular delivery of β-galactosidase enzyme was confirmed with confocal microscopy and flow cytometry analysis resulting in ∼83% co-staining of β-galactosidase enzyme and calcein AM. Based on these efficient deliveries of diverse types of biomolecules in different cell types, the device has the potential for cellular diagnostic and therapeutic applications.
1 citations
TL;DR: In this article , a one-pot surface-enhanced Raman scattering (SERS) based immunoassay was used to detect SARS-CoV-2, without any washing process using a portable Raman spectrometer.
Abstract: Rapid and sensitive diagnostics of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is of utmost importance to control the widespread coronavirus disease 2019 (COVID-19) upsurge. This study demonstrated a novel one-pot surface-enhanced Raman scattering (SERS) based immunoassay to detect SARS-CoV-2, without any washing process using a portable Raman spectrometer. The SERS-immune assay was designed using a regular digital versatile disk (DVD) substrate integrated with Raman reporter labeled silver nanoparticles for double clamping effects. The disks were molded to form nanopillar arrays and coated with silver film to enhance the sensitivity of immunoassay. The SERS platform demonstrated a limit of detection (LoD) up to 50 pg mL-1 for SARS-CoV-2 spike protein and virus-like-particle (VLP) protein in phosphate buffer saline within a turnaround time of 20 minutes. Moreover, VLP protein spiked in untreated saliva achieved an LoD of 400 pg mL-1, providing a cycle threshold (Ct) value range of 30-32, closer to reverse transcription-polymerase chain reaction (RT-PCR) results (35-40) and higher than the commercial rapid antigen tests, ranging from 25-28. Therefore, the developed one-pot SERS based biosensor exhibited highly sensitive and rapid detection of SARS-CoV-2, which could be a potential point-of-care platform for early and cost-effective diagnosis of the COVID-19 virus.
TL;DR: In this paper , a 532 nm nanosecond pulse laser and polyvinylpyrrolidone (PVP)-capped GNS were used to kill cancer cells with location-specific exposure.
Abstract: A new generation of nanoscale photosensitizer agents has improved photothermal capabilities, which has increased the impact of photothermal treatments (PTTs) in cancer therapy. Gold nanostars (GNS) are promising for more efficient and less invasive PTTs than gold nanoparticles. However, the combination of GNS and visible pulsed lasers remains unexplored. This article reports the use of a 532 nm nanosecond pulse laser and polyvinylpyrrolidone (PVP)-capped GNS to kill cancer cells with location-specific exposure. Biocompatible GNS were synthesized via a simple method and were characterized under FESEM, UV–visible spectroscopy, XRD analysis, and particle size analysis. GNS were incubated over a layer of cancer cells that were grown in a glass Petri dish. A nanosecond pulsed laser was irradiated on the cell layer, and cell death was verified via propidium iodide (PI) staining. We assessed the effectiveness of single-pulse spot irradiation and multiple-pulse laser scanning irradiation in inducing cell death. Since the site of cell killing can be accurately chosen with a nanosecond pulse laser, this technique will help minimize damage to the cells around the target cells.
TL;DR: The parallel photothermal coalescence of biocompatible photocurable polyethylene glycol diacrylate (PEGDA) droplets using a 2D image for developing a more efficient coalescence process was reported in this article .
Abstract: Droplet microfluidics is a powerful tool for high-throughput experimentation, and droplet coalescence is necessary for mixing and chemical reactions. Droplet merging and polymerization will release the limits on particle synthesis and widen the technical potential of droplet microfluidics. Previously, a focused laser beam has been used to induce the coalescence of droplets. This paper reports the parallel photothermal coalescence of biocompatible photocurable polyethylene glycol diacrylate (PEGDA) droplets using a 2D image for developing a more efficient coalescence process. PEGDA droplets with diameters of 18 µm to 50 µm were generated in a microfluidic flow-focusing device and stored in a microchannel. A 2D image of violet light induced the parallel coalescence of PEGDA droplets with diameters of around 30 µm. When continuous-phase oil was replaced with nitrogen, PEGDA droplets were photopolymerized.