Tyler Burks

TL;DR: Perturb-seq as discussed by the authors combines single-cell RNA sequencing and clustered regularly interspaced short palindromic repeats (CRISPR)-based perturbations to perform many such assays in a pool.

TL;DR: In this paper, a massively parallel single-nucleus RNA sequencing (sNuc-seq) with droplet technology is proposed. But it does not provide high throughput, and it is not suitable for high-dimensional data.

TL;DR: Perturb-seq accurately identifies individual gene targets, gene signatures, and cell states affected by individual perturbations and their genetic interactions, and posit new functions for regulators of differentiation, the anti-viral response, and mitochondrial function during immune activation.

TL;DR: To directly compare the methods and avoid processing differences introduced by the existing pipelines, scumi is developed, a flexible computational pipeline that can be used with any single-cell RNA-sequencing method.

TL;DR: This study directly compares seven methods for single cell and/or single nucleus profiling from three types of samples, generating 36 libraries in six separate experiments in a single center and developed and applied scumi, a flexible computational pipeline that can be used for any scRNA-seq method.