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Amanda J. Kedaigle
Researcher at Broad Institute
Publications - 26
Citations - 2252
Amanda J. Kedaigle is an academic researcher from Broad Institute. The author has contributed to research in topics: Huntington's disease & Organoid. The author has an hindex of 12, co-authored 21 publications receiving 1286 citations. Previous affiliations of Amanda J. Kedaigle include Massachusetts Institute of Technology & Wellesley College.
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Journal ArticleDOI
Individual brain organoids reproducibly form cell diversity of the human cerebral cortex
Silvia Velasco,Amanda J. Kedaigle,Amanda J. Kedaigle,Sean Simmons,Allison Nash,Allison Nash,Marina Rocha,Marina Rocha,Giorgia Quadrato,Giorgia Quadrato,Bruna Paulsen,Bruna Paulsen,Lan Nguyen,Xian Adiconis,Aviv Regev,Aviv Regev,Joshua Z. Levin,Paola Arlotta,Paola Arlotta +18 more
TL;DR: It is shown that an organoid model of the dorsal forebrain can reliably generate a rich diversity of cell types appropriate for the human cerebral cortex, and that establishment of terminal cell identity is a highly constrained process that can emerge from diverse stem cell origins and growth environments.
Journal ArticleDOI
Systematic comparison of single-cell and single-nucleus RNA-sequencing methods.
Jiarui Ding,Xian Adiconis,Sean Simmons,Monika S. Kowalczyk,Cynthia C. Hession,Nemanja D. Marjanovic,Travis K. Hughes,Marc H. Wadsworth,Tyler Burks,Lan T. Nguyen,John Y. Kwon,Boaz Barak,William Ge,Amanda J. Kedaigle,Shaina Carroll,Shuqiang Li,Nir Hacohen,Nir Hacohen,Orit Rozenblatt-Rosen,Alex K. Shalek,Alexandra-Chloé Villani,Alexandra-Chloé Villani,Aviv Regev,Aviv Regev,Joshua Z. Levin +24 more
TL;DR: To directly compare the methods and avoid processing differences introduced by the existing pipelines, scumi is developed, a flexible computational pipeline that can be used with any single-cell RNA-sequencing method.
Journal ArticleDOI
Huntington’s Disease iPSC-Derived Brain Microvascular Endothelial Cells Reveal WNT-Mediated Angiogenic and Blood-Brain Barrier Deficits
Ryan G. Lim,Chris Quan,Andrea M. Reyes-Ortiz,Sarah E. Lutz,Amanda J. Kedaigle,Theresa A. Gipson,Jie Wu,Gad D. Vatine,Jennifer Stocksdale,Malcolm Casale,Clive N. Svendsen,Ernest Fraenkel,David E. Housman,Dritan Agalliu,Leslie M. Thompson +14 more
TL;DR: In this article, a transcriptome and functional analysis of human induced pluripotent stem cell (iPSC)-derived brain microvascular endothelial cells (iBMECs) from HD patients or unaffected controls was performed.
Journal ArticleDOI
Developmental alterations in Huntington's disease neural cells and pharmacological rescue in cells and mice
Ryan G. Lim,Lisa Salazar,D Wilton,Alvin R. King,Jennifer Stocksdale,Delaram Sharifabad,Alice L Lau,Beth Stevens,Jack C. Reidling,Sara T. Winokur,Malcolm Casale,Leslie M. Thompson,Mónica Pardo,A Gerardo García Díaz-Barriga,Marco Straccia,Phil Sanders,Jordi Alberch,Josep M. Canals,Julia A. Kaye,Mariah Dunlap,Lisa Jo,Hanna May,Elliot Mount,Cliff Anderson-Bergman,Kelly Haston,Steven Finkbeiner,Amanda J. Kedaigle,Theresa A. Gipson,Ferah Yildirim,Christopher W. Ng,Pamela Milani,David E. Housman,Ernest Fraenkel,Nicholas D. Allen,Paul J. Kemp,Ranjit Singh Atwal,Marta Biagioli,James F. Gusella,Marcy E. MacDonald,Sergey S Akimov,Nicolas Arbez,Jacqueline Stewart,Christopher A. Ross,Virginia B. Mattis,Colton M Tom,Loren Ornelas,Anais Sahabian,Lindsay Lenaeus,Berhan Mandefro,Dhruv Sareen,Clive N. Svendsen +50 more
TL;DR: The data suggest that mutant huntingtin impairs neurodevelopmental pathways that could disrupt synaptic homeostasis and increase vulnerability to the pathologic consequence of expanded polyglutamine repeat-associated phenotypes in neural cells and of cognitive impairment and synaptic pathology in HD model R6/2 mice.
Developmental alterations in Huntington's disease neural cells and pharmacological rescue in cells and mice
Ryan G. Lim,Lisa Salazar,D Wilton,Alvin R. King,Jennifer Stocksdale,Delaram Sharifabad,L Alice Lau,Beth Stevens,Jack C. Reidling,Sara T. Winokur,Malcolm Casale,Leslie M. Thompson,Mónica Pardo,A Gerardo García Díaz-Barriga,Marco Straccia,Phil Sanders,Jordi Alberch,Josep M. Canals,Julia A. Kaye,Mariah Dunlap,Lisa Jo,Hanna May,Elliot Mount,Cliff Anderson-Bergman,Kelly Haston,Steven Finkbeiner,Nicholas D. Allen,Paul J. Kemp,Ranjit Singh Atwal,Marta Biagioli,James F. Gusella,Marcy E. MacDonald,Sergey S Akimov,Nicolas Arbez,Jacqueline Stewart,Christopher A. Ross,Virginia B. Mattis,Colton M Tom,Loren Ornelas,Anais Sahabian,Lindsay Lenaeus,Berhan Mandefro,Dhruv Sareen,Clive N. Svendsen,Amanda J. Kedaigle,Theresa Anne Wasylenko,Ferah Yildirim,Christopher W. Ng,Pamela Milani,David E. Housman,Ernest Fraenkel +50 more