Flora of Turkey and the East Aegean Islands.

It would be desirable to establish and standardize methods that can measure the total antioxidant capacity level directly from vegetable extracts containing phenolics. Antioxidant capacity assays may be broadly classified as electron transfer (ET)- and hydrogen atom transfer (HAT)-based assays. The majority of HAT assays are kinetics-based, and involve a competitive reaction scheme in which antioxidant and substrate compete for peroxyl radicals thermally generated through the decomposition of azo compounds. ET-based assays measure the capacity of an antioxidant in the reduction of an oxidant, which changes colour when reduced. ET assays include the ABTS/TEAC, CUPRAC, DPPH, Folin-Ciocalteu and FRAP methods, each using different chromogenic redox reagents with different standard potentials. This review intends to offer a critical evaluation of existing antioxidant assays applied to phenolics, and reports the development by our research group of a simple and low-cost antioxidant capacity assay for dietary polyphenols, vitamins C and E, and human serum antioxidants, utilizing the copper(II)-neocuproine reagent as the chromogenic oxidizing agent, which we haved named the CUPRAC (cupric ion reducing antioxidant capacity) method. This method offers distinct advantages over other ET-based assays, namely the selection of working pH at physiological pH (as opposed to the Folin and FRAP methods, which work at alkaline and acidic pHs, respectively), applicability to both hydrophilic and lipophilic antioxidants (unlike Folin and DPPH), completion of the redox reactions for most common flavonoids (unlike FRAP), selective oxidation of antioxidant compounds without affecting sugars and citric acid commonly contained in foodstuffs and the capability to assay -SH bearing antioxidants (unlike FRAP). Other similar ET-based antioxidant assays that we have developed or modified for phenolics are the Fe(III)- and Ce(IV)-reducing capacity methods.

/pdf/comparative-evaluation-of-various-total-antioxidant-capacity-ldu2tfjxuw.pdf

Comparative Evaluation of Various Total Antioxidant Capacity Assays Applied to Phenolic Compounds with the CUPRAC Assay

Ethnopharmacological approaches to wound healing--exploring medicinal plants of India.

The increasing incidence of drug-resistant pathogens has drawn the attention of the pharmaceutical and scientific communities towards studies on the potential antimicrobial activity of plant-derived substances, an untapped source of antimicrobial chemotypes, which are used in traditional medicine in different countries. The aim of this review is to provide recent insights regarding the possibilities of the most important natural antimicrobial compounds derived from plant sources containing a wide variety of secondary metabolites, which are useful as alternative strategies to control infectious diseases. This review will focus on natural plant products as a useful source of antimicrobial molecules, active in particular, on bacteria and fungi. When considering that many of these compounds, which have been used for centuries, are a source of new drugs and that there are ever-increasing technical breakthroughs, it can be envisaged that in the next years some different molecules discovered by ingenious screeni...

Plant-derived antimicrobial compounds: alternatives to antibiotics.

Voltammetric Techniques for the Assay of Pharmaceuticals-a Review

Antioxidant and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities, reducing powers and the amount of total phenolic compounds of aqueous and/or methanolic extracts of some medicinal plants used in Eastern Turkey were studied. These plants are Prangos ferulacea (CASIR), Sedum sempervivoides (HOROZ LELESI), Malva neglecta (EBEMGUMECI), Cruciata taurica (SARILIK OTU), Rosa pimpinellifolia (KOYUN GOZU), Galium verum subsp. verum (MADAVUR OTU), Urtica dioica (ISIRGAN). The highest peroxidation inhibitions were shown by aqueous extracts of C. taurica and R. pimpinellifolia (IC(50): 0.00022 mg/l and IC(50): 23 mg/l, respectively). However, the highest DPPH radical scavenging activity, reducing power and the amount of phenolic compounds were shown by R. pimpinellifolia. The lowest antioxidant properties were shown by aqueous extract of M. neglecta.

Antioxidant properties of some medicinal plants: Prangos ferulacea (Apiaceae), Sedum sempervivoides (Crassulaceae), Malva neglecta (Malvaceae), Cruciata taurica (Rubiaceae), Rosa pimpinellifolia (Rosaceae), Galium verum subsp. verum (Rubiaceae), Urtica dioica (Urticaceae).

In this study, antioxidant properties of Thymus sipyleus. Boiss. subsp. sipyleus. var. sipyleus., Teucrium chamaedrys L.., Mentha longifolia. (L.) Hudson subsp. longifolia., Salvia limbata. C.A. Meyer, and Thymus fallax. Fisch. & Mey. were investigated. Antioxidant and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities, reducing powers, and the amount of total phenolic compounds of the extracts were studied. The highest antioxidant activity was shown by T. chamaedrys. (decoction, IC50: 9.2 µg/ml), and the lowest one was S. limbata. (decoction, IC50: 619.5 µg/ml). The highest DPPH radical scavenging activity was shown by T. fallax. [decoction, IC50S: 56 µg/ml (IC50S is the extract concentration (µg/ml) required for 50% inhibition of the DPPH solution absorbance at 517 nm)] while the lowest one was S. limbata. (decoction, IC50S: 335.8 µg/ml). The highest reducing power and amount of total phenolic compounds was shown by T. chamaedrys. (decoction, 29.9 µg/ml ascorbic acid equivalent, ...

https://www.tandfonline.com/doi/pdf/10.1080/13880200600592061

Antioxidant Properties of Some Medicinal Lamiaceae (Labiatae) Species

Effects of Onosma armeniacum root extract on ethanol-induced oxidative stress in stomach tissue of rats.

The villages of Ilica District in Erzurum Province, Turkey, were surveyed in terms of plants and animals used traditionally for medicinal purposes. Plants were compiled according to their families, botanical names, local names, usages, and applications. A total of 70 plant species belonging to 29 families are used by village people to treat a wide range of complaints, such as hemorrhoids, urinary system infections, stomach disorders, respiratory diseases, skin diseases, rheumatism, and jaundice. In addition, 3 animal species are used for the treatment of some diseases. The collected data were compared with those formerly recorded.

/pdf/folk-medicines-in-the-villages-of-ilica-district-erzurum-2k1c5d3rh2.pdf

Folk medicines in the villages of Ilıca District (Erzurum, Turkey)

Relationship between chemical structure and antioxidant activity of luteolin and its glycosides isolated from Thymus sipyleus subsp. sipyleus var. sipyleus

Ufuk Özgen

Papers

Antioxidant properties of some medicinal plants: Prangos ferulacea (Apiaceae), Sedum sempervivoides (Crassulaceae), Malva neglecta (Malvaceae), Cruciata taurica (Rubiaceae), Rosa pimpinellifolia (Rosaceae), Galium verum subsp. verum (Rubiaceae), Urtica dioica (Urticaceae).

Antioxidant Properties of Some Medicinal Lamiaceae (Labiatae) Species

Effects of Onosma armeniacum root extract on ethanol-induced oxidative stress in stomach tissue of rats.

Folk medicines in the villages of Ilıca District (Erzurum, Turkey)

Relationship between chemical structure and antioxidant activity of luteolin and its glycosides isolated from Thymus sipyleus subsp. sipyleus var. sipyleus