Utham K. Valekunja

TL;DR: It is shown that oxidation–reduction cycles of peroxiredoxin proteins constitute a universal marker for circadian rhythms in all domains of life, by characterizing their oscillations in a variety of model organisms and exploring the interconnectivity between these metabolic cycles and transcription–translation feedback loops of the clockwork in each system.

TL;DR: Unexpectedly, in Bmal1 knockout mice, both tissues exhibited 24-hour oscillations of the transcriptome, proteome, and phosphoproteome over 2 to 3 days in the absence of any exogenous drivers such as daily light or temperature cycles, suggesting that such oscillations might be underpinned by transcriptional regulation by the recruitment of ETS family transcription factors, and nontranscriptionally by co-opting redox oscillations.

TL;DR: The pentose phosphate pathway (PPP), a critical source of the redox cofactor NADPH, is identified as an important regulator of redox and transcriptional oscillations, suggesting a pivotal role for NADPH availability in circadian timekeeping.

TL;DR: Circadian modification of an activating histone mark at a significant proportion of gene loci that undergo daily transcription is demonstrated, implicating widespread epigenetic modification as a key node regulated by the clockwork.

TL;DR: This work applied low-amplitude temperature cycles to synchronize populations of animals through development and reveals rhythms on multiple levels: in olfactory cued behavior, in RNA and protein abundance, and in the oxidation state of a broadly conserved peroxiredoxin protein.