V
Vasilica Nache
Researcher at University of Jena
Publications - 21
Citations - 621
Vasilica Nache is an academic researcher from University of Jena. The author has contributed to research in topics: Cyclic nucleotide-gated ion channel & Gating. The author has an hindex of 11, co-authored 21 publications receiving 546 citations.
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Journal ArticleDOI
Relating ligand binding to activation gating in CNGA2 channels
Christoph Biskup,Jana Kusch,Eckhard Schulz,Vasilica Nache,Frank Schwede,Frank Lehmann,Volker Hagen,Klaus Benndorf +7 more
TL;DR: The action of homotetrameric olfactory-type CNGA2 channels was studied in inside-out membrane patches by simultaneously determining channel activation and ligand binding, using the fluorescent cGMP analogue 8-DY547-cGMP as the ligand.
Journal ArticleDOI
Interdependence of Receptor Activation and Ligand Binding in HCN2 Pacemaker Channels
Jana Kusch,Christoph Biskup,Susanne Thon,Eckhard Schulz,Vasilica Nache,Thomas Zimmer,Frank Schwede,Klaus Benndorf +7 more
TL;DR: It is shown that full ligand-induced activation appears already with only two ligands bound to the tetrameric channel, proving thus the principle of reciprocity between ligand binding and conformational change in a receptor protein.
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Coumarinylmethyl esters for ultrafast release of high concentrations of cyclic nucleotides upon one- and two-photon photolysis.
Volker Hagen,Brigitte Dekowski,Vasilica Nache,Reinhard Schmidt,Daniel Geissler,Dorothea Lorenz,Jenny Eichhorst,Sandro Keller,Hiroshi Kaneko,Klaus Benndorf,Burkhard Wiesner +10 more
Journal ArticleDOI
How subunits cooperate in cAMP-induced activation of homotetrameric HCN2 channels
Jana Kusch,Susanne Thon,Eckhard Schulz,Christoph Biskup,Vasilica Nache,Thomas Zimmer,Reinhard Seifert,Frank Schwede,Klaus Benndorf +8 more
TL;DR: This analysis of the complex activation mechanism of homotetrameric HCN2 channels by confocal patch-clamp fluorometry and kinetically quantify all ligand binding steps and closed-open isomerizations of the intermediate states suggests a dimeric organization of the activation process.
Journal ArticleDOI
Activation of olfactory-type cyclic nucleotide-gated channels is highly cooperative.
Vasilica Nache,Eckhard Schulz,Thomas Zimmer,Jana Kusch,Christoph Biskup,Rolf Koopmann,Volker Hagen,Klaus Benndorf +7 more
TL;DR: It is shown that at equal degree of activation, the activation time course of homotetrameric channels is similar with cGMP and cAMP and it is also similar in homo‐ and heterotet Krameric channels with the same cyclic nucleotide.