V
Volker Oberle
Researcher at University of Groningen
Publications - 10
Citations - 3202
Volker Oberle is an academic researcher from University of Groningen. The author has contributed to research in topics: Transfection & Gene delivery. The author has an hindex of 7, co-authored 10 publications receiving 2997 citations.
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Size-dependent internalization of particles via the pathways of clathrin-and caveolae-mediated endocytosis
TL;DR: The data indicate that the size itself of (ligand-devoid) particles can determine the pathway of entry in non-phagocytic B16 cells, and kinetic parameters may determine the almost exclusive internalization of such particles along this pathway rather than via caveolae.
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Fluorescent lipid probes : some properties and applications (a review)
TL;DR: A brief overview of the application of several lipid probes in cell biological and biophysical research, and critically analyze their validity in the various fields is provided.
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Lipoplex Formation under Equilibrium Conditions Reveals a Three-Step Mechanism
TL;DR: This work has studied the interaction between a plasmid and cationic amphiphiles, using a monolayer technique, and has examined complex assembly by atomic force microscopy to reveal a three-step mechanism for complex formation.
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Phase behavior of cationic amphiphiles and their mixtures with helper lipid influences lipoplex shape, DNA translocation, and transfection efficiency
Inge S. Zuhorn,Volker Oberle,Willy H. Visser,Jan B.F.N. Engberts,Udo Bakowsky,Evgeny Polushkin,Dick Hoekstra +6 more
TL;DR: A structure-function study with two pyridinium-based lipid analogs with identical headgroups but differing in alkyl chain (un)saturation found a role of the structural shape of the plasmid in gaining cytosolic/nuclear access and proposed that such a translocation is promoted when the lipoplex adopts the hexagonal phase.
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The influence of physicochemical parameters on the efficacy of non-viral DNA transfection complexes: a comparative study.
Carsten Kneuer,Carsten Ehrhardt,Heike Bakowsky,M. N. V. Ravi Kumar,Volker Oberle,Claus M. Lehr,Dick Hoekstra,Udo Bakowsky +7 more
TL;DR: Transfection efficiency seems to require a positive or neutral zeta potential, which is depending on size, e.g., is higher for smaller particles, and requires a vector that is stable in serum.