Showing papers by "W. Hayes McDonald published in 2001"
TL;DR: It is reported that Mus81 and an associated protein Eme1 are components of an endonuclease that resolves Holliday junctions into linear duplex products and constitute strong evidence that Mus 81 and Eme 1 are subunits of a nuclear Holliday junction resolvase.
534 citations
TL;DR: Several known binding proteins co‐purified with both CDC2p‐CTAP and N‐TAP‐Cdc2p, indicating the usefulness of these tags for the rapid purification of stable protein complexes from Sz.
Abstract: We describe the construction of tagging cassettes and plasmids for tandem affinity purification (TAP) of proteins in Schizosaccharomyces pombe. The tagging cassettes are designed for either carboxy- or amino-terminal tagging of proteins. The carboxyl terminal tags differ in that they contain either two or four repeats of IgG binding units. For tagging endogenous loci, the cassettes contain the kan MX6 module to allow for selection of G418-resistant cells. The amino-terminal tagging vectors allow for the regulated expression of proteins. Sz. pombe Cdc2p was chosen to test these new affinity tags. Several known binding proteins co-purified with both Cdc2p-CTAP and N-TAP-Cdc2p, indicating the usefulness of these tags for the rapid purification of stable protein complexes from Sz. pombe. Copyright © 2001 John Wiley & Sons, Ltd.
154 citations