Wan Hon Koh

TL;DR: The objective of this work was to create a database, namely LTDB, with a significant number of manually tracked leukocytes to foster the development of robust computer vision techniques for imaging-based immunological research.

TL;DR: P perturbation of the actin cytoskeleton via the lentiviral protein Nef, and not changes to chemokine receptor expression or function, is the dominant cause of dysregulated infected T cell motility in lymphoid tissue by preventing stable cellular polarization required for fast migration.

TL;DR: It is shown that HIV-infected macrophages can engage T cells in stable contacts through binding of virus- and host-derived adhesive molecules and that stable macrophage-T cell contacts were required for high viral spread.

TL;DR: A mechanism by which surface bound HIV-1 particles function as signaling receptors that regulate T cell motility, cell-cell contact dynamics, and productive infection is described.

TL;DR: A detailed protocol to study cells within an all-in-one 3D collagen matrix that is amenable to live-cell microscopy and immunohistochemistry is presented, which facilitates analyses of dynamic cellular events in 3D settings.