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Y. V. Sheludko
Researcher at National Academy of Sciences of Ukraine
Publications - 26
Citations - 569
Y. V. Sheludko is an academic researcher from National Academy of Sciences of Ukraine. The author has contributed to research in topics: Rauvolfia serpentina & Gene. The author has an hindex of 13, co-authored 24 publications receiving 519 citations. Previous affiliations of Y. V. Sheludko include Technische Universität Darmstadt & University of Mainz.
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Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression.
TL;DR: It is displayed that N. excelsior has the best characteristics in regard to biomass yield as well as GFP accumulation level for both types of the expression cassettes tested, and usage of viral-based expression system resulted in considerable increase of G FP accumulation in N. benthamiana and N. exigua.
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Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids.
TL;DR: From the data it can be concluded that the divergence of the biosynthetic pathways leading to different classes of indole alkaloids formed in R. serpentina and C. roseus cell suspension cultures occurs at a later stage than strictosidine deglucosylation.
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High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic-electrospray ionisation mass spectrometric analysis of selected alkaloid groups.
Joachim Stöckigt,Y. V. Sheludko,Y. V. Sheludko,Matthias Unger,Irina Gerasimenko,Irina Gerasimenko,Heribert Warzecha,Detlef Stöckigt +7 more
TL;DR: The developed conditions for CE analysis proved to be efficient for separation of mixtures of standard indole and beta-carboline alkaloids and the described buffer system is also applicable in the combination of CE with electrospray ionisation mass spectrometry.
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New alkaloids of the sarpagine group from Rauvolfia serpentina hairy root culture.
TL;DR: Three new monoterpenoid indole alkaloids were isolated from hairy root culture of Rauvolfia serpentina, and their structures were elucidated by 1D and 2D NMR analyses.
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3-Oxo-rhazinilam: A New Indole Alkaloid from Rauvolfia serpentina × Rhazya stricta Hybrid Plant Cell Cultures
TL;DR: A new monoterpenoid indole alkaloid was isolated from intergeneric somatic hybrid cell cultures of Rauvolfia serpentina and Rhazya stricta and it was proved that 3-oxo-rhazinilam is a natural constituent of the hybrid cells.