Heribert Warzecha

TL;DR: The use of edible plants for the production and delivery of vaccine proteins could provide an economical alternative to fermentation systems, but the technology is limited by low expression levels for nuclear-integrated transgenes, but recent progress in plant organelle transformation shows promise for enhanced expression.

TL;DR: A standard for Type IIS restriction endonuclease-mediated assembly is described, defining a common syntax of 12 fusion sites to enable the facile assembly of eukaryotic transcriptional units.

TL;DR: It is shown that full-length L1 protein can be expressed and localized in plant cell nuclei and that expression of L1 in plants is enhanced by removal of the carboxy-terminal nuclear localization signal sequence, and ingestion of such material can activate potentially protective humoral immune responses.

TL;DR: Extraordinarily high expression levels and the prospects of developing edible biopharmaceuticals make transgenic chloroplasts a promising platform for the production of next-generation vaccines and antimicrobials.

TL;DR: The results indicate that an oral unadjuvanted vaccine based on U-Omp19 induces protection against a mucosal challenge with Brucella abortus by inducing an adaptive IL-17 immune response and indicate different and important new aspects i) IL- 17 does not contribute to reduce the bacterial burden in non vaccinated mice and ii) IL -17 plays a central role in vaccine mediated anti-Brucella mucosal immunity.