Y
Yasuaki Shirayoshi
Researcher at Tottori University
Publications - 76
Citations - 1729
Yasuaki Shirayoshi is an academic researcher from Tottori University. The author has contributed to research in topics: Embryonic stem cell & Chromosome 21. The author has an hindex of 20, co-authored 72 publications receiving 1588 citations.
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Journal ArticleDOI
Proteomics-based identification of differentially expressed genes in human gliomas: Down-regulation of SIRT2 gene
Masaharu Hiratsuka,Toshiaki Inoue,Tosifusa Toda,Narimichi Kimura,Yasuaki Shirayoshi,Hideki Kamitani,Takashi Watanabe,Eisaku Ohama,Candice G.T. Tahimic,Akihiro Kurimasa,Mitsuo Oshimura +10 more
TL;DR: Results suggest that SIRT2 may act as a tumor suppressor gene in human glioma possibly through the regulation of microtubule network and may serve as a novel molecular marker for gliomas.
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Targeted disruption of the human LIT1 locus defines a putative imprinting control element playing an essential role in Beckwith-Wiedemann syndrome.
Shin-ichi Horike,Kohzoh Mitsuya,Makiko Meguro,Noriko Kotobuki,Akiko Kashiwagi,Tomomi Notsu,Thomas C. Schulz,Yasuaki Shirayoshi,Mitsuo Oshimura +8 more
TL;DR: It is demonstrated that the Lit1 CpG island can act as a negative regulator in cis for coordinate imprinting at the centromeric domain, thereby suggesting a role for the LIT1 locus in a BWS pathway leading to functional inactivation of p57(KIP2).
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Refined human artificial chromosome vectors for gene therapy and animal transgenesis.
Yasuhiro Kazuki,Hidetoshi Hoshiya,Masato Takiguchi,Satoshi Abe,Yuichi Iida,Mitsuhiko Osaki,Motonobu Katoh,Masaharu Hiratsuka,Yasuaki Shirayoshi,Kei Hiramatsu,E Ueno,Naoyo Kajitani,Toko Yoshino,Kanako Kazuki,Chie Ishihara,Shoko Takehara,Saori Tsuji,F Ejima,Atsushi Toyoda,Yoshiyuki Sakaki,Vladimir Larionov,Natalay Kouprina,Mitsuo Oshimura +22 more
TL;DR: This study refined a HAC vector without endogenous genes from human chromosome 21 in homologous recombination-proficient chicken DT40 cells, and demonstrated that any desired gene can be cloned into the HAC using the Cre-loxP system in Chinese hamster ovary cells, or a homologously recombination system inDT40 cells.
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A highly stable and nonintegrated human artificial chromosome (HAC) containing the 2.4 Mb entire human dystrophin gene.
Hidetoshi Hoshiya,Yasuhiro Kazuki,Satoshi Abe,Masato Takiguchi,Naoyo Kajitani,Yoshinori Watanabe,Toko Yoshino,Yasuaki Shirayoshi,Katsumi Higaki,Graziella Messina,Giulio Cossu,Mitsuo Oshimura +11 more
TL;DR: The development of a HAC vector containing the entire human dystrophin gene (DYS-HAC) that is stably maintained in mice and human immortalized mesenchymal stem cells (hiMSCs) is reported.
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Construction of a novel human artificial chromosome vector for gene delivery.
Motonobu Katoh,Fumiaki Ayabe,Satoko Norikane,Teruaki Okada,Hiroshi Masumoto,Shin-ichi Horike,Yasuaki Shirayoshi,Mitsuo Oshimura,Mitsuo Oshimura +8 more
TL;DR: A novel HAC from sequence-ready human chromosome 21 by telomere-directed chromosome truncation is developed and a loxP sequence is added for site-specific insertion of circular DNA by the Cre/loxP system, suggesting that the HAC vector provides useful system for functional studies of genes in isogenic cell lines.