Y
Yasuo Niwa
Researcher at University of Shizuoka
Publications - 50
Citations - 6159
Yasuo Niwa is an academic researcher from University of Shizuoka. The author has contributed to research in topics: Arabidopsis & Gene. The author has an hindex of 23, co-authored 49 publications receiving 5756 citations.
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Development of series of gateway binary vectors, pGWBs, for realizing efficient construction of fusion genes for plant transformation
Tsuyoshi Nakagawa,Takayuki Kurose,Takeshi Hino,Katsunori Tanaka,Makoto Kawamukai,Yasuo Niwa,Kiminori Toyooka,Ken Matsuoka,Tetsuro Jinbo,Tetsuya Kimura +9 more
TL;DR: A new series of binary vectors useful for Gateway cloning to facilitate transgenic experiments in plant biotechnology realized efficient cloning, constitutive expression using the cauliflower mosaic virus (CaMV) 35S promoter and the construction of fusion genes by simple clonase reaction with an entry clone.
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Engineered GFP as a vital reporter in plants
TL;DR: It is reported that an extensively modified GFP is a versatile and sensitive reporter in a variety of living plant cells and in transgenic plants, and the codon usage effect might be universal, allowing the design of recombinant proteins with high expression efficiency in evolutionarily distant species such as humans and maize.
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Improved Gateway binary vectors: high-performance vectors for creation of fusion constructs in transgenic analysis of plants.
Tsuyoshi Nakagawa,Takamasa Suzuki,Satoko Murata,Shinya Nakamura,Takeshi Hino,Kenichiro Maeo,Ryo Tabata,Tsutae Kawai,Katsunori Tanaka,Yasuo Niwa,Yuichiro Watanabe,Kenzo Nakamura,Tetsuya Kimura,Sumie Ishiguro +13 more
TL;DR: Improved pGWBs should be a powerful tool for transgenic research in plants because of their small size and high-copy number in Escherichia coli make possible easier handling at plasmid preparation and sequencing.
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Green-fluorescent protein as a new vital marker in plant cells
TL;DR: This new reporter could be used to monitor gene expression, signal transduction, co-transfection, transformation, protein trafficking and localization, protein-protein interaction, cell separation and purification, and cell lineage in higher plants.
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Non-invasive quantitative detection and applications of non-toxic, S65T-type green fluorescent protein in living plants
TL;DR: In this article, a non-invasive, quantitative detection system was proposed for S65T-type GFP mutants, one of the brightest chromophore mutants among the various GFPs.