Yasuo Niwa

TL;DR: A new series of binary vectors useful for Gateway cloning to facilitate transgenic experiments in plant biotechnology realized efficient cloning, constitutive expression using the cauliflower mosaic virus (CaMV) 35S promoter and the construction of fusion genes by simple clonase reaction with an entry clone.

TL;DR: It is reported that an extensively modified GFP is a versatile and sensitive reporter in a variety of living plant cells and in transgenic plants, and the codon usage effect might be universal, allowing the design of recombinant proteins with high expression efficiency in evolutionarily distant species such as humans and maize.

TL;DR: Improved pGWBs should be a powerful tool for transgenic research in plants because of their small size and high-copy number in Escherichia coli make possible easier handling at plasmid preparation and sequencing.

TL;DR: This new reporter could be used to monitor gene expression, signal transduction, co-transfection, transformation, protein trafficking and localization, protein-protein interaction, cell separation and purification, and cell lineage in higher plants.

TL;DR: In this article, a non-invasive, quantitative detection system was proposed for S65T-type GFP mutants, one of the brightest chromophore mutants among the various GFPs.