Although understudied in academia and mostly unheard of by the general public, the in situ/ex situ dichotomy has shaped — and still very much shapes — the development of the nature conservation movement and its institutional alliances in the last few decades. Latin for “in” and “out” of place, the in/ex situ dichotomy often stands for the seemingly less scientific dichotomy between wild nature and captivity. Drawing on ethnographic engagements with zoo professionals and wildlife managers, this article explores the evolution of the in situ/ex situ dyad in nature conservation, which traverses the worlds of dead and live matter, artificilia and naturalia, and the seemingly disconnected institutions of museums and zoos, game parks, and nature reserves. Drawing on animal and relational geography, the article suggests that the assumptions underlying the in situ versus ex situ divide in conservation are anachronistic, romantic, and unsustainable and that they are incompatible with ideas of naturecultures and multinatures and with non-traditional perceptions of space. Eventually, this grounded study of conservation discourses and practices highlights the possibility of conservation management without nature.

Conservation without Nature: The Trouble with In Situ versus Ex Situ Conservation

Green synthesis of silver nanoparticles from aqueous extract of Ctenolepis garcini L. and assess their possible biological applications

Abstract Australia is home to over 800 different species of Eucalyptus and traditionally, many Eucalyptus species have been utilised to heal wounds and treat fungal infections by the Indigenous people of Australia. In view of this, our study was designed to investigate the phytochemical, antibacterial and antifungal properties of crude aqueous extract of E . microcorys leaves. The freeze-dried powdered extract was prepared and the phytochemical profile was studied by analysing the total phenolic content (TPC), total flavonoid content (TFC), proanthocyanidins, antioxidants and saponins. The TPC, TFC and proanthocyanidin values found were: 501.76 ± 14.47 mg of gallic acid equivalents per g, 61.53 ± 0.83 mg of rutin equivalents per g and 10.76 ± 0.89 mg of catechin equivalents per g, respectively. The antioxidant values expressed in mg trolox equivalents per g of extract (mg TE/g) were: ABTS = 1073.13 ± 10.73 mg TE/g, DPPH = 1035.44 ± 65.54 mg TE/g and CUPRAC = 1524.30 ± 66.43 mg TE/g. The powdered extract was also evaluated for activity against three pathogenic bacterial strains ( Escherichia coli , Enterobacter aerogenes , Staphylococcus lugdunensis ); and three fungal strains ( Geotrichum candidum , Aspergillus brasiliensis and Candida albicans ) using the disc diffusion method and 96 well plate-based method with resazurin dye. The extract exhibited clear zones of inhibition against the tested bacteria and fungi. Minimum inhibitory concentration (MIC) values were demonstrated to be: A . brasiliensis  = 2.44 μg/mL, G . candidum  = 4.88 μg/mL, S . lugdunensis  = 78 μg/mL, E . coli  = 156.25 μg/mL, E . aerogenes  = 312.5 μg/mL and C . albicans  = 1250 μg/mL. These results reveal the significant potential of E . microcorys as a source of phenolics, antioxidants and antimicrobial agents and also highlight the necessity of further purification and characterisation of solitary bioactive compounds for their prospective applications in food, nutraceutical and pharmaceutical industries.

Phytochemical, antibacterial and antifungal properties of an aqueous extract of Eucalyptus microcorys leaves

スギ(Cryptomeria japonica)黒心材の殺蟻成分

Nutmeg ( Myristica fragrans Houtt ) is an important spices in Indonesia. The advantages of nutmeg is essential oil content, the result of distillation which is for the spices industry, the manufacture of soaps, perfumes, cosmetics, have high economic value and are a source of foreign exchange to non oil & gas, Indonesia is able to supply the needs of the market the world of up to 70% to 75%. Generally, nutmeg in Indonesia use generative propagation which has the strong root system and long life, but the germination takes a long time. Using the natural plant growth regulators for germination can be used to accelerate growth. Natural PGR’s can be extracted from some plants such shallot as a source of auxin, banana hump as a source of cytokinins, and bamboo shoots as a source of giberellin.The purpose of this study was to determine the types of natural PGR’s that have good effect on the growth of nutmeg seedling. The experiment was carried out in Desa, Bugel, Kecamatan Ciawi Tasikmalaya with altitude at 600 meters above sea level, from June 2018 to September 2018. This study used Randomized Completely Block Design (RCBD) Method. The treatments were: a0= control, a1= shallot, a2= bamboo shoot, a3= banana hump, a4= shallot + bamboo shoot, a5= shallot +, banana hump, a6= bamboo shoot + banana hump, a7= shallot + bamboo shoot + banana hump.The result showed that:1) type of natural substances plant growth regulator effected on various growth variable, 2) Combination of shallot bulb + bamboo shoot, or combination of shallot bulb + bamboo shoot and banana hump good effected on plant height, seedling diametre, number of leaf, leaf area, and shoot root ratio.

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Effect of Type of Natural Substances Plant Growth Regulator on Nutmeg ( Myristica Fragrans ) Seedlings

. Nurlaeni Y, Iskandar J, Junaedi DI. 2021. Ethnoecology of Zanthoxylum acanthopodium by local communities around Lake Toba, North Sumatra, Indonesia. Biodiversitas 22: 1806-1818. Zanthoxylum acanthopodium DC., locally known as andaliman, is a typical spice of traditional cuisine of communities living around Lake Toba, North Sumatra, Indonesia. Despite the extensive uses, the ethnoecological study on this plant is relatively scarce. This study aims to conduct ethnoecological studies of the management and cultivation of andaliman plants based on Traditional Ecological Knowledge of local communities of Salaon Dolok Village, a village nearby Lake Toba which has close relationship and intensively cultivated/utilized andaliman in their daily life. This study applied qualitative methods using the combination of direct observation and interviews with 46 selected respondents using snowball sampling. The results of this study showed that the cultivation of andaliman in Salaon Dolok Village was carried out using traditional ways with simple farming practices. These traditional farming models relied on manual farming practices in all farming aspects. The community has local knowledge related to the management of reliable agricultural systems from generation to generation. Andaliman cultivation in Salaon Dolok Village does not need maintenance costs for fertilizers and pesticides. In general, andaliman planting is mostly done in the forest. In general, the people of this village only recognize two kinds of andaliman cultivars, namely Andaliman 'Simanuk' and Andaliman 'Sihorbo'. The planting is usually carried out during the rainy season to reduce the mortality rate of the planted seedlings. The activity of seedling preparation is carried out in two simple ways. First, most farmers were collected andaliman seeds that grow wild around the existing andaliman plants. The second method is by collecting the seedling that grows from the burning land with some remains of old andaliman plants collected at the edge of the land. Sustainable agriculture should minimize the external input from outside of the agro-ecosystem. Low External Input Sustainable Agriculture (LEISA) emphasizes the efficient use of existing production factors to create sustainable agriculture, including for andaliman agriculture system.

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Ethnoecology of Zanthoxylum acanthopodium by local communities around Lake Toba, North Sumatra, Indonesia

. Pratiwi RA, Nurlaeni Y. 2020. Screening of plant collection of Cibodas Botanic Gardens, Indonesia with anticancer properties. Biodiversitas 21: 5186-5229. Cancer is a life-threatening disease worldwide. One approach to developing effective treatment in fighting cancerous cells is to obtain anticancer drug candidates from natural resources, such as plants. This study aimed to inventory and categorize plant collections in Cibodas Botanic Gardens (CBG), West Java, Indonesia that has anticancer properties in a detailed and comprehensive manner. Literature research was conducted in international scientific databases using several keywords expressing anticancer properties to produce list of plant species potential for anticancer. The results of this research were then cross-checked with the plant collection database of CBG. List of plants exhibits anticancer activities were then categorized based on the IC50 values (an indicator of cytotoxicity). Our result showed 291 species from 90 families of CBG plant collection harbor anticancer properties. Among them, 93, 100, 36, and 62 species have IC50 values under Class I (strong), Class II (moderate), Class III (inactive), and Class IV (insufficient IC50 data), respectively. The families with the highest number of potential anticancer plants are Lauraceae, Leguminosae, Meliaceae, Myrtaceae, Moraceae, Cupressaceae, Asparagaceae, Euphorbiaceae, Compositae, Clusiaceae, Lamiaceae, Apocynaceae, Adoxaceae, Amaryllidaceae, and Elaeocarpaceae. Species that have strong anticancer activities include Acacia farnesiana, Aglaia edulis, A. elliptica, A. silvestris, Artocarpus elasticus, Bauhinia strychnifolia, Buxus microphylla, Calophyllum soulattri, Cerbera manghas, Cocculus orbiculatus, Cryptocarya chinensis, C. konishii, C. laevigata, Dalbergia parviflora, Diospyros discolor, Erythrina abyssinica, Etlingera elatior, Ficus fistulosa, Garcinia x mangostana, Hemerocallis fulva, Jatropha gossypiifolia, Panax ginseng, Podocarpus macrophyllus, Psidium cattleianum, Sansevieria ehrenbergii, Tacca chantrieri, Toona sinensis, Viburnum odoratissimum, and V. Sambucinum. Even Serenoa repens and Taxus sumatrana contain active compounds that have been commercialized as anticancer drugs. The data resulted from this study can serve as baseline information for further research in drug discovery and development for anticancer treatments using living plant specimens collected in CBG. CBG has a great prospect of medicinal plants that require further studies for formulating anticancer drug as an alternative natural resource.

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Screening of plant collection of Cibodas Botanic Gardens, Indonesia with anticancer properties

Nurlaeni Y, Surya MI. 2015. Response of Camelia japonica shoot cuttings on organic Plant Growth Regulator. Pros Sem Nas Masy Biodiv Indon 1: 1211-1215. Camelia japonica L. is a herbaceous plant which has the potential as ornamental and medicinal plants. Application of plant growth regulators (PGR) is an effort to increase the quality of seedling through vegetative propagation. However, a good quality of organic PGR relatively has not been developed yet. This study aimed to examine the provision of organic plant growth regulator on the growth of Camelia japonica shoot cuttings. The experiment was arranged in a completely randomized design with 5 replications. The factors were various concentration of organic PGR such as 0%, 5%, 25%, 50%, 100%. Rootone-F with a dose 2 g/mL was used for comparison. The observed growth parameters were the percentage of live cuttings, root number, root length, stem diameter, callus diameter, number of flowers, wet weight of plant and dry weight of plant. The results showed that there is no significant difference between organic and synthetic PGR. The difference is only visible on plant fresh weight parameters, with the highest value in the treatment of 25% organic PGR. It indicates that the organic PGR has the same ability with synthetic PGR to stimulate the growth of Camelia japonica shoot cuttings.

Respon stek pucuk Camelia japonica terhadap pemberian Zat Pengatur Tumbuh organik

Mount Seblat, as part a of Kerinci Seblat National Park (KSNP), is a pristine and natural mountain, particularly from disturbances and destructions by human activities. Nevertheless, the richness of biological resources especially plant diversity.in this area has not been more explored. The purpose of this study was to conduct an inventory of plant diversity and to determine the plant species composition. The inventory activities were conducted by plants collection along the ascent route. The results were then be maintained through ex situ conservation method in Cibodas Botanical Garden (CBG). The study was conducted by exploratory method, from Seblat Ulu Village (641 m asl) up to altitude of 1,037 m asl. There were 18 points of plant sample observation with an area of 5 x 5 square meters per point. Plant collection obtained 380 specimens. Five groups of most collected plants were Lauraceae (18 species), Rubiaceae (8 species), Anacardiaceae (6 species), Annonaceae (5 species), and Fagaceae (4 species). In order to enrich the plants collection as well as conduct the ex situ conservation effort, plants from Orchidaceae were also collected which resulted in 33 species. These results were an important initial inventory of plant diversity of Mount Seblat, considering that there was no record as well as very limited current information. When the environment disturbance tends to increase, this information may act as a reference and an initial database to develop plants conservation effort and strategy in the future.

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Ex situ Conservation Effort through the Inventory of Plant Diversity in Mount Seblat, Bengkulu

. Pratiwi RA, Nurlaeni Y. 2021. The potency of Myrtaceae Family from Cibodas Botanic Gardens (Cianjur, Indonesia) as botanical pesticide. Biodiversitas 22: 4648-4664. Cibodas Botanic Gardens (CBG) is a biodiversity reservoir that can be explored for the discovery of new candidates for botanical pesticides. Myrtaceae has been reported to provide biological activity against pests or pathogens due to their essential oil contents. This research was conducted to inventory and categorize Myrtaceae collection in CBG that had the potency to be botanical pesticide. The list of Myrtaceae collection of CBG obtained from Unit Registration and Collection CBG per 2021. Database fulfilling regarding the bactericide/fungicide/herbicide/insecticide potency of the Myrtaceae of CBG’s collection was carried out through digital references search. Our result showed that there were 73 species of Myrtaceae (from 18 genera) that are potential to be botanical pesticide sources. There were 17 species that are considerably had a high potency. Most of them belong to the Eucalyptus and Melaleuca, followed by Backhousia, Leptospermum, Psidium, and Syzygium. The data resulted from this study is expected to serve as baseline information for further research about the formulation, efficacy, and conservation management of botanical pesticides from Myrtaceae for sustainable use. Furthermore, the development of biological pesticides is a step to improve the quality of Indonesian export products so as to increase national competitiveness in the globalization era nowadays.

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Yati Nurlaeni

Papers

Ethnoecology of Zanthoxylum acanthopodium by local communities around Lake Toba, North Sumatra, Indonesia

Screening of plant collection of Cibodas Botanic Gardens, Indonesia with anticancer properties

Respon stek pucuk Camelia japonica terhadap pemberian Zat Pengatur Tumbuh organik

Ex situ Conservation Effort through the Inventory of Plant Diversity in Mount Seblat, Bengkulu

The potency of Myrtaceae Family from Cibodas Botanic Gardens (Cianjur, Indonesia) as botanical pesticide