Yiider Tseng

TL;DR: This paper introduces the method of live-cell multiple-particle-tracking microrheology (MPTM), which quantifies the local mechanical properties of living cells by monitoring the Brownian motion of individual microinjected fluorescent particles, and investigates the mechanical function of alpha-actinin in non-muscle cells.

TL;DR: Both the mechanical properties of the cytOSkeleton and cytoskeleton-based processes, including cell motility, coupled MTOC and nucleus dynamics, and cell polarization, depend critically on the integrity of the nuclear lamina, which suggest the existence of a functional mechanical connection between the nucleus and the cytos skeleton.

TL;DR: A model of strain hardening for F-actin networks is proposed, based on both the intrinsic rigidity of F-Actin and dynamic topological constraints formed by the cross-linkers located at filaments entanglements, which offers an explanation for the origin of strainhardening observed when shear stresses are applied against the cellular membrane.

TL;DR: A lower bound of the propulsive forces required for nuclear organelles such as promyelocytic-leukemia bodies to undergo processive transport within the nucleus is determined by overcoming friction forces set by the intranuclear viscosity.

TL;DR: By monitoring the displacement of well-dispersed microspheres via fluorescence microscopy, the degree of spatial heterogeneity of F-actin gels and networks in vitro is probed and multiple-particle tracking offers a new, quantitative method to characterize the organization of biopolymers in solution.